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HDAC2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423756

MBS423756 is an antibody targeting Hdac2. Also known as Goat anti-Hdac2 (mouse) Antibody. Reactive in HumanExpected from sequence similarity: Mouse, Rat, Dog, Pig, Cow. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hdac2, Antibody
Full Product Name
Goat anti-Hdac2 (mouse) Antibody
Product Gene Name
anti-Hdac2 antibody
Product Synonym Names
Hdac2; histone deacetylase 2; D10Wsu179e; YAF1; Yy1bp; mRPD3; HD2; OTTMUSP00000022803; YY1 transcription factor-binding protein
Species Reactivity
Human
Expected from sequence similarity: Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Hdac2 peptide (MBS428794) is used for blocking the activity of Hdac2 antibody (MBS423756)
Molecular Weight
55.3kDa
3D Structure
ModBase 3D Structure for P70288
Immunogen Sequence
Peptide with sequence C-PEDAVHEDSGDE, from the internal region of the protein sequence according to NP_032255.2.
Peptide Sequence + C
C-PEDAVHEDSGDE
Epitope
Internal Region
Note
This antibody imay cross-react with HDAC1
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-Hdac2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-Hdac2 antibody
Pep-ELISA: antibody detection limit dilution 1:2000.
WB: Approx 55-60kDa band observed in nuclear lysates of cell line HEK293. Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.
IF: Strong expression of the protein seen in the nucleus of U251 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Immunohistochemistry (IHC) of anti-Hdac2 antibody

(1ug/ml) staining of HEK293 nuclear cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.
Anti-HDAC2 Antibody – Cat# MBS423756

Immunofluorescence (IF) of anti-Hdac2 antibody

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Anti-HDAC2 Antibody, Immunofluorescence (IF) – Cat# MBS423756

Flow Cytometry (FC/FACS) of anti-Hdac2 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Anti-HDAC2 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS423756

Other Notes

Small volumes of anti-Hdac2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hdac2. It may not necessarily be applicable to this product.

NCBI GI #
87162464
NCBI GeneID
15182
NCBI Accession #
NP_032255.2
UniProt Primary Accession #
P70288
UniProt Secondary Accession #
B2RRP3
UniProt Related Accession #
P70288
GenBank Nucleotide #
NM_008229.2
NCBI Official Full Name
histone deacetylase 2
NCBI Official Synonym Full Names
histone deacetylase 2
NCBI Official Symbol
Hdac2
NCBI Official Synonym Symbols
YAF1; Yy1bp; mRPD3; D10Wsu179e
UniProt Protein Name
Histone deacetylase 2
UniProt Synonym Protein Names
YY1 transcription factor-binding protein
UniProt Gene Name
Hdac2
UniProt Synonym Gene Names
Yy1bp; HD2
NCBI Protein Information
histone deacetylase 2

UniProt Comments for Hdac2

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
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Research Articles on Hdac2

1. these findings suggested that HDAC2 may be an important negative regulator involved in chronic stressinduced cognitive impairment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hdac2 antibody

 Reference Product  PubMed Publications
 Hdac5 antibody  >48 publications with Hdac2 and Hdac5
 Sin3a antibody  >43 publications with Hdac2 and Sin3a
 Hdac9 antibody  >16 publications with Hdac2 and Hdac9
 Rbbp4 antibody  >14 publications with Hdac2 and Rbbp4
 Mta2 antibody  >12 publications with Hdac2 and Mta2
 Chd4 antibody  >12 publications with Hdac2 and Chd4
 Sin3b antibody  >8 publications with Hdac2 and Sin3b
 Ncor2 antibody  >8 publications with Hdac2 and Ncor2
 Rbbp7 antibody  >8 publications with Hdac2 and Rbbp7
 Sap30 antibody  >6 publications with Hdac2 and Sap30

Pathways associated with anti-Hdac2 antibody

 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Signaling Pathway antibodies  Cell Cycle Signaling Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with anti-Hdac2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >189 publications with Hdac2 and Inflammation
 Pulmonary Disease, Chronic Obstructive Antibodies  >120 publications with Hdac2 and Pulmonary Disease, Chronic Obstructive
 Cardiovascular Diseases Antibodies  >72 publications with Hdac2 and Cardiovascular Diseases
 Heart Diseases Antibodies  >66 publications with Hdac2 and Heart Diseases
 Adenocarcinoma Antibodies  >63 publications with Hdac2 and Adenocarcinoma
 Necrosis Antibodies  >61 publications with Hdac2 and Necrosis
 Breast Neoplasms Antibodies  >44 publications with Hdac2 and Breast Neoplasms
 Fibrosis Antibodies  >41 publications with Hdac2 and Fibrosis
 Leukemia, Myeloid Antibodies  >37 publications with Hdac2 and Leukemia, Myeloid
 Liver Diseases Antibodies  >37 publications with Hdac2 and Liver Diseases

Organs/Tissues associated with anti-Hdac2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >234 publications with Hdac2 and Brain
 Lung Antibodies  >208 publications with Hdac2 and Lung
 Blood Antibodies  >150 publications with Hdac2 and Blood
 Heart Antibodies  >132 publications with Hdac2 and Heart
 Muscle Antibodies  >96 publications with Hdac2 and Muscle
 Embryonic Tissue Antibodies  >34 publications with Hdac2 and Embryonic Tissue
 Kidney Antibodies  >30 publications with Hdac2 and Kidney
 Skin Antibodies  >25 publications with Hdac2 and Skin
 Intestine Antibodies  >25 publications with Hdac2 and Intestine
 Bone Marrow Antibodies  >20 publications with Hdac2 and Bone Marrow
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