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SOX2 Antibody – Goat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423840

MBS423840 is an antibody targeting SOX2. Also known as Goat Anti-SOX2 Antibody. Reactive in HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SOX2, Antibody
Full Product Name
Goat Anti-SOX2 Antibody
Product Gene Name
Product Synonym Names
SOX2; SRY (sex determining region Y)-box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2
Host
Goat
Species Reactivity
Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
SOX2 peptide (MBS428878) is used for blocking the activity of SOX2 antibody (MBS423840)
OMIM
184429
3D Structure
ModBase 3D Structure for P48431
Immunogen
Peptide with sequence YNMMETELKPPGPQQ-C, from the N Terminus of the protein sequence according to NP_003097.1.
Peptide Sequence
YNMMETELKPPGPQQ
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SOX2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-SOX2 antibody
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3kDa according to )NP_003097.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Western Blot (WB) of anti-SOX2 antibody

(0.3µg/ml) staining of MCF7 (A) HEK293 (B) and MCF7 nuclear (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Goat Anti-SOX2 Antibody – Cat# MBS423840

Flow Cytometry (FC) of anti-SOX2 antibody

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Goat Anti-SOX2 Antibody, Flow Cytometry (FC) – Cat# MBS423840

Immunohistochemistry (IHC) of anti-SOX2 antibody

(8ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-SOX2 Antibody, Immunohistochemistry (IHC) – Cat# MBS423840

Immunohistochemistry (IHC) of anti-SOX2 antibody

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Goat Anti-SOX2 Antibody, Immunohistochemistry (IHC) – Cat# MBS423840

Product References and Citations for anti-SOX2 antibody

Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD.: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80. PMID: 15849174

Other Notes

Small volumes of anti-SOX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY-box transcription factor 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
NCBI Protein Information
transcription factor SOX-2

NCBI Summary for SOX2

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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