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POU4F2 Antibody – Goat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423854

MBS423854 is an antibody targeting POU4F2. Also known as Goat Anti-POU4F2 Antibody. Reactive in HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
POU4F2, Antibody
Full Product Name
Goat Anti-POU4F2 Antibody
Product Gene Name
anti-POU4F2 antibody
Product Synonym Names
POU4F2; POU class 4 homeobox 2; BRN3.2; BRN3B; Brn-3b; Brn3b POU domain transcription factor; POU domain class 4 transcription factor 2; POU domain protein; POU domain, class 4, transcription factor 2
Host
Goat
Species Reactivity
Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
OMIM
113725
3D Structure
ModBase 3D Structure for Q12837
Immunogen
Peptide with sequence C-EKSHREKLTKPE, from the internal region of the protein sequence according to NP_004566.2.
Peptide Sequence
EKSHREKLTKPE
Epitope
Internal region
Important comments
This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-POU4F2 antibody
Peptide ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-POU4F2 antibody
Peptide ELISA: antibody detection limit dilution 1:1000
FC/FACS: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Flow Cytometry (FC/FACS) of anti-POU4F2 antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
Goat Anti-POU4F2 Antibody – Cat# MBS423854

Product References and Citations for anti-POU4F2 antibody

Qiu F, Jiang H, Xiang M.: A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors. The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 28 (13): 3392-403. PMID: 18367606

Other Notes

Small volumes of anti-POU4F2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU4F2. It may not necessarily be applicable to this product.

NCBI GI #
110347455
NCBI GeneID
5458
NCBI Accession #
NP_004566.2
UniProt Primary Accession #
Q12837
UniProt Related Accession #
Q12837
GenBank Nucleotide #
NP_004566.2
NCBI Official Full Name
POU domain, class 4, transcription factor 2
NCBI Official Synonym Full Names
POU class 4 homeobox 2
NCBI Official Symbol
POU4F2
NCBI Official Synonym Symbols
BRN3B; BRN3.2; Brn-3b
UniProt Protein Name
POU domain, class 4, transcription factor 2
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 3B; Brain-3B; Brn-3B
Protein Family
POU domain, class 4, transcription factor
UniProt Gene Name
POU4F2
UniProt Synonym Gene Names
BRN3B; Brain-3B; Brn-3B
UniProt Entry Name
PO4F2_HUMAN
NCBI Protein Information
POU domain, class 4, transcription factor 2

NCBI Summary for POU4F2

The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. [provided by RefSeq, Sep 2011]
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UniProt Comments for POU4F2

POU4F2: Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q31.2

Cellular Component: nuclear speck; nucleus

Molecular Function: chromatin binding

Biological Process: neuron differentiation; axon guidance; estrogen receptor signaling pathway; sensory perception of sound; transcription, DNA-dependent; retina development in camera-type eye; axon extension; MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; neuromuscular process controlling balance; positive regulation of cell differentiation; retinal ganglion cell axon guidance
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Research Articles on POU4F2

1. DNA methylation in a combination of POU4F2/PCDH17 has yielded the highest sensitivity and specificity of 90.00% and 93.96% in all the 312 individuals, showing the capability of detecting bladder cancer effectively among pathologically varied sample groups.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POU4F2 antibody

 Reference Product  PubMed Publications
 ATOH7 antibody  >12 publications with POU4F2 and ATOH7
 PAX6 antibody  >7 publications with POU4F2 and PAX6
 BAX antibody  >2 publications with POU4F2 and BAX
 EBF1 antibody  >1 publications with POU4F2 and EBF1
 TP53 antibody  >1 publications with POU4F2 and TP53
 HSPB1 antibody  >1 publications with POU4F2 and HSPB1
 ATOH1 antibody  >1 publications with POU4F2 and ATOH1
 DLX1 antibody  >1 publications with POU4F2 and DLX1

Pathways associated with anti-POU4F2 antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Regulation Of TP53 Activity Pathway antibodies  Regulation Of TP53 Activity Pathway Diagram
 Regulation Of TP53 Activity Through Association With Co-factors Pathway antibodies  Regulation Of TP53 Activity Through Association With Co-factors Pathway Diagram
 Transcriptional Regulation By TP53 Pathway antibodies  Transcriptional Regulation By TP53 Pathway Diagram

Diseases associated with anti-POU4F2 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >9 publications with POU4F2 and Disease Models, Animal
 Embryo Loss Antibodies  >3 publications with POU4F2 and Embryo Loss
 Brain Diseases Antibodies  >2 publications with POU4F2 and Brain Diseases
 Uterine Cervical Neoplasms Antibodies  >2 publications with POU4F2 and Uterine Cervical Neoplasms
 Congenital Abnormalities Antibodies  >2 publications with POU4F2 and Congenital Abnormalities
 Kidney Diseases Antibodies  >1 publications with POU4F2 and Kidney Diseases
 Immune System Diseases Antibodies  >1 publications with POU4F2 and Immune System Diseases
 Edema Antibodies  >1 publications with POU4F2 and Edema
 Hyperglycemia Antibodies  >1 publications with POU4F2 and Hyperglycemia
 Atrophy Antibodies  >1 publications with POU4F2 and Atrophy

Organs/Tissues associated with anti-POU4F2 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >58 publications with POU4F2 and Eye
 Testis Antibodies  >1 publications with POU4F2 and Testis
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