Olfactomedin 4 (OLFM4) ELISA Kit for Human

Name: Human OLFM4 ELISA Kit; Olfactomedin 4; MyBioSource
Brand: MyBioSource
SKU: MBS042405
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS042405

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Olfactomedin 4 (OLFM4), ELISA Kit

Full Product Name

Human Olfactomedin 4 ELISA Kit

Product Gene Name

OLFM4 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

6.25 ng/ml - 200 ng/ml.

Sensitivity

1.0 ng/ml.

Specificity

No significant cross-reactivity or interference between Human OLFM4 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

57,280 Da

Chromosome Location

Chromosome: 13; NC_000013.10 (53602876..53626196). Location: 13q14.3

OMIM

614061

3D Structure

ModBase 3D Structure for Q6UX06

Other Notes

Small volumes of OLFM4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OLFM4 purchase

MBS042405 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Olfactomedin 4 (OLFM4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OLFM4. The ELISA analytical biochemical technique of the MBS042405 kit is based on OLFM4 antibody-OLFM4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OLFM4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OLFM4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for OLFM4. It may not necessarily be applicable to this product.

NCBI GI #

32313593

NCBI GeneID

10562

NCBI Accession #

NP_006409.3

UniProt Primary Accession #

Q6UX06

UniProt Secondary Accession #

O95362; Q5VWG0; Q86T22

UniProt Related Accession #

Q6UX06

GenBank Nucleotide #

NM_006418.4

NCBI Official Full Name

olfactomedin-4

NCBI Official Synonym Full Names

olfactomedin 4

NCBI Official Symbol

OLFM4

NCBI Official Synonym Symbols

GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1

UniProt Protein Name

Olfactomedin-4

UniProt Synonym Protein Names

Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; hGC-1; hOLfD

Protein Family

Olfactomedin

UniProt Gene Name

OLFM4

UniProt Synonym Gene Names

GW112; OLM4; hGC-1

UniProt Entry Name

OLFM4_HUMAN

NCBI Protein Information

olfactomedin-4; antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein

NCBI Summary for OLFM4

This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. [provided by RefSeq, Mar 2011]

UniProt Comments for OLFM4

OLFM4: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: extracellular space; specific granule; azurophil granule; mitochondrion; perinuclear region of cytoplasm; plasma membrane; secretory granule

Molecular Function: protein homodimerization activity; cadherin binding; catalytic activity

Biological Process: regulation of apoptosis; metabolic process; negative regulation of immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; cell adhesion; regulation of phagocytosis; protein homooligomerization

Research Articles on OLFM4

1. OLFM4 is overexpressed in active IBD and secreted into mucus. OLFM4 seems to be of functional relevance in IBD as a mucus component, possibly by binding defensins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OLFM4 elisa kit

Reference Product	PubMed Publications
LGR5 elisa kit	>10 publications with OLFM4 and LGR5
DEFA4 elisa kit	>1 publications with OLFM4 and DEFA4
NOD2 elisa kit	>1 publications with OLFM4 and NOD2
NOD1 elisa kit	>1 publications with OLFM4 and NOD1

Diseases associated with OLFM4 elisa kit

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>9 publications with OLFM4 and Adenocarcinoma
Kidney Diseases Antibodies	>4 publications with OLFM4 and Kidney Diseases
Prostatic Neoplasms Antibodies	>2 publications with OLFM4 and Prostatic Neoplasms
Skin Diseases Antibodies	>2 publications with OLFM4 and Skin Diseases
Prostatic Diseases Antibodies	>2 publications with OLFM4 and Prostatic Diseases
Lung Neoplasms Antibodies	>2 publications with OLFM4 and Lung Neoplasms
Cerebral Infarction Antibodies	>1 publications with OLFM4 and Cerebral Infarction
Growth Disorders Antibodies	>1 publications with OLFM4 and Growth Disorders
Chromosome Aberrations Antibodies	>1 publications with OLFM4 and Chromosome Aberrations
Pulmonary Fibrosis Antibodies	>1 publications with OLFM4 and Pulmonary Fibrosis

Organs/Tissues associated with OLFM4 elisa kit

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>15 publications with OLFM4 and Stomach
Intestine Antibodies	>11 publications with OLFM4 and Intestine
Prostate Antibodies	>5 publications with OLFM4 and Prostate
Kidney Antibodies	>4 publications with OLFM4 and Kidney
Nerve Antibodies	>2 publications with OLFM4 and Nerve
Cervix Antibodies	>1 publications with OLFM4 and Cervix
Testis Antibodies	>1 publications with OLFM4 and Testis
Pancreas Antibodies	>1 publications with OLFM4 and Pancreas
Uterus Antibodies	>1 publications with OLFM4 and Uterus