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TAQ Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS430074

Anti-Taq monoclonal antibodies were derived from a hybridoma (fusion of mouse myeloma cell and the cells after mouse immunization with Taq DNA Polymerase).Anti-Taq monoclonal antibody inhibits polymerase activity before the onset of thermal cycling,… More details

$210 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Taq, Monoclonal Antibody
Full Product Name
Anti-Taq Monoclonal Antibody (clone 8C1)
Product Gene Name
Product Synonym Names
Anti-Taq Monoclonal Antibody; Anti-Taq Monoclonal Antibody
Clonality
Monoclonal
Clone Number
8C1
Form/Format
10 mM Tris-HCl (pH 7.0), 50 mM KCl, 0.1 mM EDTA, 50% Glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
100ug / 1mg (4mg/ml) (lot specific)
Preparation and Storage
For long term storage , store at -20°C , stable up to 1 year. For immediate use, keep at 4°C , stable for up to one month.
Reaction Buffer
The anti-Taq monoclonal antibody reaction buffer is the same buffer used for the thermostable DNA polymerase.
Associated Activities
No conversion to the covalently closed circular DNA to the nicked or linear form was observed after incubation of 1 ug of pUC19 with antibodies in final concentration of 2.4 ug/ul in 20 ul of reaction mixture containing 25 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2 after 16 hours at 37°C.
Note
For each Taq DNA polymerase or Taq from different batch, the antibody titration test must be performed to find the best ratio of antibody to Taq DNA polymerase. Too much antibody added to Taq DNA polymerase could kill the PCR reaction
Protocol
1. Before making any dilution of anti-Taq antibody and Taq polymerase, add 1 ug anti-Taq monoclonal antibody to 2 ug Taq DNA polymerase (~20 Units).
2. Mix gently and incubate at RT for about 10-20 minutes or store at 4°C.
3. Set up the PCR reaction by following the protocol of a regular thermal cycling condition used for theTaq DNA polymerase.

Product Categories/Family for anti-Taq antibody

Applications Tested/Suitable for anti-Taq antibody
- Real-time or regular hot start PCR applications;
- PCR diagnostics, genotyping, SNP etc.

Other Notes

Small volumes of anti-Taq antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Taq purchase

MBS430074 is an antibody targeting Taq. Also known as Anti-Taq Monoclonal Antibody (clone 8C1).
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