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Enterokinase (rEK) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS430250

Recombinant Enterokinase (rEK) is the catalytic subunit of bovine enterokinase, which is expressed by the yeast Pichia pastoris and purified to yield a high enzyme activity preparation. rEK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide… More details

$520 / 1000 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Enterokinase (rEK), Recombinant Protein
Full Product Name
Recombinant Enterokinase (rEK)
Product Gene Name
rEK recombinant protein
Product Synonym Names
Recombinant Enterokinase
Host
Yeast
Preparation and Storage
Store at -20 degree C
Unit Definition
One unit of rEK is the amount of enzyme that will cleave 20ug of thioredoxin-chloramphenicci acetyl transferase fusion protein containing an enterokinase cleavage site (Asp-Asp-Asp-Asp-Lys) to 90% completion at 37 degree C in 16 hours
Assay Conditions
Recombinant EK in 50mM Tris-HCl, pH 8.0, 1mM CaCl2, 0.1% Tween-20, 20ug of fusion protein, and 1 unit rEK in a 30ul reaction volume incubated at 37 degree C.
Non-Specific Protease Activity Assay
A non-specific protease activity assay of rEK was performed using azocasein as substrate. The results show that rEK contains less than background levels of non-specific protease.
Storage Buffer
rEK in 50mM PBNa, pH 8.0, 0.5M NaCl and 50% glycerol

Product Categories/Family for rEK recombinant protein

Cytokine Proteins

Other Notes

Small volumes of rEK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rEK purchase

MBS430250 is a recombinant protein targeting rEK. Also known as Recombinant Enterokinase (rEK). Host: Yeast.
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