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EZ-Safe DNA Reagent

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS433668

Description: EZ Safe-Stain is an environment safe non-EB nucleic acid stain used for detecting double-stranded DNA in agarose gels. EZ Safe-Stain is supplied in 6x DNA loading buffer which contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and… More details

$195 / 1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EZ-Safe DNA, Reagent
Full Product Name
EZ-Safe DNA Stain Buffer
Product Synonym Names
EZ Safe-Stain DNA Loading Buffer; EZ Safe-Stain DNA Stain Buffer
Preparation and Storage
Store at 4 degree C in dark, stable for one year from the date of shipment. For long term storage: -20 degree C.
Note
1) The thickness of gel could affect the gel sensitivity.
2) EZ Safe-Stain allows visualization of DNA in the agarose gel under blue light. This eliminates the need for exposure to UV light, which can nick and damage DNA. The intact DNA fragments purified from agarose gel can increase the efficiency of subsequent molecular biology manipulations such as cloning, sequencing, transformation and transcription.
Protocol
1. Prepare agarose gel in 1x TBE or 1x TAE (or use a precast agarose gel).
2. Mix well the 6x EZ Safe-Stain DNA loading buffer prior to use.
3. Add 1x EZ Safe-Stain DNA loading buffer to DNA samples or DNA ladders, and then proceed directly to sample loading.
4. Run the agarose gel.
5. Monitor the DNA migration under blue light or with a blue light monitor.
6. Document the image under the UV light. We recommend not using UV light if the DNA fragments are to be used for subsequent cloning or sequencing.
Contents
1 ml (6x) in DNA loading buffer.

Product Categories/Family for EZ-Safe DNA reagent

Reagents and Buffer
Application Notes for EZ-Safe DNA reagent
Visualization of DNA bands as they separate during agarose gel electrophoresis using blue light; Isolation of DNA fragments for subclonning without introducing mutations normally caused by EB and ultraviolet.

Testing Data of EZ-Safe DNA reagent

EZ-Safe DNA Reagent – Cat# MBS433668

Testing Data of EZ-Safe DNA reagent

EZ-Safe DNA Reagent, Testing Data – Cat# MBS433668

Other Notes

Small volumes of EZ-Safe DNA reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EZ-Safe DNA purchase

MBS433668 is a reagent targeting EZ-Safe DNA. Also known as EZ-Safe DNA Stain Buffer.
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