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5x SDS Reagent

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS433670

MBS433670 is a reagent targeting 5x SDS. Also known as 5x SDS Protein Sample Loading Buffer. More details

$135 / 10 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
5x SDS, Reagent
Full Product Name
5x SDS Protein Sample Loading Buffer
Form/Format
5x SDS protein sample loading buffer: 250 mM Tris-Hel pHS,8, 10% SDS, 30% Glycerol, 0.25% Bromophenol blue.
Preparation and Storage
Room temperature
PROTOCOL FOR REDUCING SDS-PAGE
For 20 ul sample loading volume
• Add 4 ul 5x SDS protein sample loading buffer, 2 ul 10x Protein sample reducing reagent, appropriate amount of protein (20-50 ug for cell lysate; 20-50 ng for purified protein), ddH20 to final volume.
• Heat samples at 95°C for 2 minutes.
• Cool on ice, spin down and load onto SDS-PAGE gel.
Usage
This product is only for laboratory research and development

Product Categories/Family for 5x SDS reagent

Applications Tested/Suitable for 5x SDS reagent
SDS-PAGE
Application Notes for 5x SDS reagent
• Ready to use for non-reducing SDS-PAGE;
• For reducing SOS-PAGE, add 1x protein sample reducing reagent.

Other Notes

Small volumes of 5x SDS reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for 5x SDS. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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