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Anti-Ha-Tag Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435049

Anti-HA-Tag Mouse mAb (Agarose Conjugated) is a monoclonal anti-HA antibody covalently linked to agarose; the agarose enables immunoprecipitation (IP) of HA tagged proteins or co-immunoprecipitation (Co-IP) of their interacting partners. More details

$520 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Anti-HA-Tag, Monoclonal Antibody
Full Product Name
Anti-HA-Tag mAb (Agarose conjugated)
Product Synonym Names
Anti-HA-Tag Mouse mAb (Agarose Conjugated)
Clonality
Monoclonal
Isotype
Mouse IgG1
Host
Mouse
Species Reactivity
General
Specificity
Anti-HA-Tag Mouse mAb detects transfected proteins containing the HA epitope tag.
Preparation and Storage
The product is supplied as a 50% slurry in storage buffer (1x PBS, pH 7.4, containing 0.1% NaN3). Store the product at 4 degree C and do not freeze.
Source Note
This monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).
Negative Control
Mouse negative control serum MBS435004 is suggested as a blocking agent or negative control in immunoassays

Product Categories/Family for anti-Anti-HA-Tag antibody

Beads-antibody; Conjugated Antibodies

Western Blot (WB) of anti-Anti-HA-Tag antibody

HEK 293T cells were transfected with HA-tagged protein or not, and 100 ul cell lysate (about 100 ug total protein) was incubated with 30 ul 50% slurry of Anti-HA Agarose for 3 h at 4 degree C. After washing, the beads were eluted by 30 ul elution buffer twice. After neutralization of the eluant, 6 ul 6x SDS loading buffer was added. Then 20 ul sample was subjected to the SDS-PAGE. Blot was probed with Anti-HA-Tag Mouse mAb.
Lane 1: 1st Elution with elution buffer.
Lane 2: IP of untransfected HEK 293T lysate.
Mouse Anti-Ha-Tag Monoclonal Antibody – Cat# MBS435049

Other Notes

Small volumes of anti-Anti-HA-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Anti-HA-Tag purchase

MBS435049 is an antibody targeting Anti-HA-Tag. Also known as Anti-HA-Tag mAb (Agarose conjugated). Reactive in General. Host: Mouse.
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