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Myc-Tag Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435061

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag… More details

$275 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Myc-Tag, Monoclonal Antibody
Full Product Name
Myc-Tag (19C2) Mouse mAb
Clonality
Monoclonal
Isotype
Mouse IgG1
Host
Mouse
Species Reactivity
All
Specificity
Myc-Tag (19C2) Mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Source Note
This monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Product Categories/Family for anti-Myc-Tag antibody

Applications Tested/Suitable for anti-Myc-Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
Application Notes for anti-Myc-Tag antibody
WB: 1:5000
IF: 1:2000
IP: 1:200
ELISA: 1:2000
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.05% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-Myc-Tag antibody

Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of cell lysate.
Mouse Anti-Myc-Tag Monoclonal Antibody – Cat# MBS435061

Immunofluorescence (IF) of anti-Myc-Tag antibody

IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (right, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution.
Mouse Anti-Myc-Tag Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS435061

Immunoprecipitation (IP) of anti-Myc-Tag antibody

IP of extracts from 293T cells transfected (lane 1) with a Myc-tagged protein and a mock transfection (lane 2, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:200 (1), 1:500 (2), 1:200 (3).
Mouse Anti-Myc-Tag Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435061

Western Blot (WB) of anti-Myc-Tag antibody

Western Blot analysis of over-expressed Myc-tagged protein in 293T cell lysate using Myc-Tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane1) and 1:10000 (lane 2). Each lane was loaded with 10ug of cell lysate.
Mouse Anti-Myc-Tag Monoclonal Antibody, Western Blot (WB) – Cat# MBS435061

Product References and Citations for anti-Myc-Tag antibody

Munro, S. and Pelham, H.R. 1984. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J. 3, 3087-3093.

Other Notes

Small volumes of anti-Myc-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Myc-Tag purchase

MBS435061 is an antibody targeting Myc-Tag. Also known as Myc-Tag (19C2) Mouse mAb. Reactive in All. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Myc-Tag. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
MYC-tag, partial

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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