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PCCA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435089

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PCCA, Monoclonal Antibody
Full Product Name
PCCA Mouse mAb
Product Gene Name
anti-PCCA antibody
Product Synonym Names
alpha polypeptide; mitochondrial; pccA complementation group; PCCase alpha subunit; PCCase subunit alpha; propanoyl-CoA:carbon dioxide ligase alpha subunit; Propanoyl-CoA:carbon dioxide ligase subunit alpha; propionyl CoA carboxylase, alpha polypeptide; propionyl Coenzyme A carboxylase; propionyl Coenzyme A carboxylase, alpha polypeptide; propionyl-CoA carboxylase alpha chain; Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCA
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 80kDa
3D Structure
ModBase 3D Structure for P05165
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human PCCA

Product Categories/Family for anti-PCCA antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-PCCA antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-PCCA antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-PCCA antibody

Mouse Anti-PCCA Monoclonal Antibody – Cat# MBS435089

Immunoprecipitation (IP) of anti-PCCA antibody

PCCA was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25077 of 10 ug. Western blot was performed from the immunopre-cipitate using M25077 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 80 KDa
Observed band size : 80 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-PCCA Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435089

Flow Cytometry (FC/FACS) of anti-PCCA antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PCCA with M25077 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-PCCA Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435089

Other Notes

Small volumes of anti-PCCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCCA purchase

MBS435089 is an antibody targeting PCCA. Also known as PCCA Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PCCA. It may not necessarily be applicable to this product.

NCBI GI #
65506442
NCBI GeneID
5095
NCBI Accession #
NP_000273.2
UniProt Primary Accession #
P05165
UniProt Secondary Accession #
Q15979; Q8WXQ7; B4DKY8; B4DPF9; C9JPQ8
UniProt Related Accession #
P05165
GenBank Nucleotide #
NM_000282.3
NCBI Official Full Name
propionyl-CoA carboxylase alpha chain, mitochondrial isoform a
NCBI Official Synonym Full Names
propionyl-CoA carboxylase subunit alpha
NCBI Official Symbol
PCCA
UniProt Protein Name
Propionyl-CoA carboxylase alpha chain, mitochondrial
UniProt Synonym Protein Names
Propanoyl-CoA:carbon dioxide ligase subunit alpha
UniProt Gene Name
PCCA
UniProt Synonym Gene Names
PCCase subunit alpha
NCBI Protein Information
propionyl-CoA carboxylase alpha chain, mitochondrial

Research Articles on PCCA

1. The majority of patients had mutations in the PCCA gene (18/25). A total of 26 mutations were noted: 20 in the PCCA gene and 6 in PCCB gene. Seventeen mutations were novel (14 in PCCA and 3 in PCCB). The SNP c.937C>T (p.Arg313Ter), was noted in 9/36 (25%) alleles in the PCCA gene

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PCCA antibody

 Reference Product  PubMed Publications
 PCCB antibody  >53 publications with PCCA and PCCB
 ACAA2 antibody  >1 publications with PCCA and ACAA2
 MCEE antibody  >1 publications with PCCA and MCEE

Pathways associated with anti-PCCA antibody

 Products by Pathway  Pathway Diagram
 2-oxobutanoate Degradation Pathway antibodies  2-oxobutanoate Degradation Pathway Diagram
 2-oxobutanoate Degradation I Pathway antibodies  2-oxobutanoate Degradation I Pathway Diagram
 Biotin Transport And Metabolism Pathway antibodies  Biotin Transport And Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram

Diseases associated with anti-PCCA antibody

 Disease Name  Pubmed Publications
 Propionic Acidemia Antibodies  >53 publications with PCCA and Propionic Acidemia
 Carcinoma Antibodies  >7 publications with PCCA and Carcinoma
 Adenocarcinoma Antibodies  >7 publications with PCCA and Adenocarcinoma
 Fatty Liver Antibodies  >6 publications with PCCA and Fatty Liver
 Disease Models, Animal Antibodies  >6 publications with PCCA and Disease Models, Animal
 Liver Diseases Antibodies  >4 publications with PCCA and Liver Diseases
 Liver Neoplasms Antibodies  >4 publications with PCCA and Liver Neoplasms
 Atrophy Antibodies  >4 publications with PCCA and Atrophy
 Seizures Antibodies  >3 publications with PCCA and Seizures
 Heart Diseases Antibodies  >3 publications with PCCA and Heart Diseases

Organs/Tissues associated with anti-PCCA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >43 publications with PCCA and Blood
 Liver Antibodies  >21 publications with PCCA and Liver
 Vascular Antibodies  >7 publications with PCCA and Vascular
 Skin Antibodies  >6 publications with PCCA and Skin
 Heart Antibodies  >5 publications with PCCA and Heart
 Muscle Antibodies  >5 publications with PCCA and Muscle
 Brain Antibodies  >5 publications with PCCA and Brain
 Nerve Antibodies  >2 publications with PCCA and Nerve
 Placenta Antibodies  >2 publications with PCCA and Placenta
 Embryonic Tissue Antibodies  >2 publications with PCCA and Embryonic Tissue
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