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EF1A3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435095

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EF1A3, Monoclonal Antibody
Full Product Name
EF1A3 Mouse mAb
Product Gene Name
Product Synonym Names
eEF1A-like 3
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 50kDa
3D Structure
ModBase 3D Structure for Q5VTE0
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human EF1A3
Applications Tested/Suitable for anti-EF1A3 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-EF1A3 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.05% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-EF1A3 antibody

Mouse Anti-EF1A3 Monoclonal Antibody – Cat# MBS435095

Immunoprecipitation (IP) of anti-EF1A3 antibody

EF1A3 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25068 of 10 ug. Western blot was performed from the immunopre-cipitate using M25068 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 50 KDa
Observed band size : 50 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-EF1A3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435095

Flow Cytometry (FC/FACS) of anti-EF1A3 antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling EF1A3 with M25068 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-EF1A3 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435095

Other Notes

Small volumes of anti-EF1A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EF1A3 purchase

MBS435095 is an antibody targeting EF1A3. Also known as EF1A3 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for EF1A3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Putative elongation factor 1-alpha-like 3
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 1 pseudogene 5
NCBI Official Symbol
EEF1A1P5
NCBI Official Synonym Symbols
EEF1AL3
UniProt Protein Name
Putative elongation factor 1-alpha-like 3
UniProt Synonym Protein Names
Eukaryotic elongation factor 1 A-like 3; eEF1A-like 3; Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5
UniProt Gene Name
EEF1A1P5
UniProt Synonym Gene Names
EEF1AL3; EF-1-alpha-like 3; eEF1A-like 3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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