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ATP5A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435116

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked… More details

$435 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
ATP5A, Monoclonal Antibody
Full Product Name
ATP5A (027) Mouse mAb
Product Gene Name
anti-ATP5A antibody
Product Synonym Names
alpha subunit; ATP synthase; ATP synthase alpha chain; ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5AL2; ATPM; cardiac muscle; epididymis secretory sperm binding protein Li 123m; H+ transporting; hATP1; isoform 1; mitochondrial; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial F1 complex; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2; MOM2; OMR; ORM; AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATPM; BOS_22238; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 59kDa
3D Structure
ModBase 3D Structure for P25705
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human ATP5A

Product Categories/Family for anti-ATP5A antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-ATP5A antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-ATP5A antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-ATP5A antibody

All lanes : ATPA Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 59 KDa Observed band size : 59 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-ATP5A Monoclonal Antibody – Cat# MBS435116

Immunoprecipitation (IP) of anti-ATP5A antibody

ATPA was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25014 of 10 ug. Western blot was performed from the immunoprecipitate using M25014 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2:IP product of Jurkat cells membrane fraction.
Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-ATP5A Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435116

Flow Cytometry (FC/FACS) of anti-ATP5A antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ATPA with M25014 at 1/200 dilution (yellow) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-ATP5A Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435116

Other Notes

Small volumes of anti-ATP5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATP5A purchase

MBS435116 is an antibody targeting ATP5A. Also known as ATP5A (027) Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ATP5A. It may not necessarily be applicable to this product.

NCBI GI #
50345982
NCBI GeneID
498
NCBI Accession #
NP_001001935.1
UniProt Primary Accession #
P25705
UniProt Secondary Accession #
Q53XX6; Q8IXV2; Q96FB4; Q96HW2; Q96IR6; Q9BTV8; A8K092; B4DY56; K7ENP3
UniProt Related Accession #
P25705
GenBank Nucleotide #
NM_001001935.2
NCBI Official Full Name
ATP synthase subunit alpha, mitochondrial isoform c
NCBI Official Synonym Full Names
ATP synthase F1 subunit alpha
NCBI Official Symbol
ATP5F1A
NCBI Official Synonym Symbols
OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
UniProt Protein Name
ATP synthase subunit alpha, mitochondrial
UniProt Synonym Protein Names
ATP synthase F1 subunit alpha
UniProt Gene Name
ATP5F1A
UniProt Synonym Gene Names
ATP5A; ATP5A1ImportedManual assertion inferred from database entriesiHGNC:823; ATP5AL2ImportedManual assertion inferred from database entriesiHGNC:823; ATPMImportedManual assertion inferred from database entriesiHGNC:823
NCBI Protein Information
ATP synthase subunit alpha, mitochondrial

UniProt Comments for ATP5A

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites ().
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Research Articles on ATP5A

1. Using systems biology techniques to study gene coexpression network, ATP5A1 was identified and found highly expressed in normal kidney than clear cell renal cell carcinoma (ccRCC) tissues of each grade. Functional and pathway enrichment analysis demonstrated that ATP5A1 is overrepresented in pathway of oxidative phosphorylation, which associated with tumorigenesis and tumor progression.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATP5A antibody

 Reference Product  PubMed Publications
 ATP5C1 antibody  >2 publications with ATP5A and ATP5C1

Pathways associated with anti-ATP5A antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram
 F-type ATPase, Eukaryotes Pathway antibodies  F-type ATPase, Eukaryotes Pathway Diagram
 Formation Of ATP By Chemiosmotic Coupling Pathway antibodies  Formation Of ATP By Chemiosmotic Coupling Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mitochondrial Protein Import Pathway antibodies  Mitochondrial Protein Import Pathway Diagram

Diseases associated with anti-ATP5A antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with ATP5A and Neoplasms
 Breast Neoplasms Antibodies  >2 publications with ATP5A and Breast Neoplasms
 Carcinoma Antibodies  >2 publications with ATP5A and Carcinoma
 Death Antibodies  >1 publications with ATP5A and Death
 Immune System Diseases Antibodies  >1 publications with ATP5A and Immune System Diseases
 Lung Neoplasms Antibodies  >1 publications with ATP5A and Lung Neoplasms
 Nervous System Diseases Antibodies  >1 publications with ATP5A and Nervous System Diseases
 Drug Toxicity Antibodies  >1 publications with ATP5A and Drug Toxicity
 Kidney Diseases Antibodies  >1 publications with ATP5A and Kidney Diseases
 Inflammation Antibodies  >1 publications with ATP5A and Inflammation

Organs/Tissues associated with anti-ATP5A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with ATP5A and Brain
 Ovary Antibodies  >3 publications with ATP5A and Ovary
 Liver Antibodies  >3 publications with ATP5A and Liver
 Blood Antibodies  >2 publications with ATP5A and Blood
 Skin Antibodies  >2 publications with ATP5A and Skin
 Bone Antibodies  >2 publications with ATP5A and Bone
 Pancreas Antibodies  >1 publications with ATP5A and Pancreas
 Muscle Antibodies  >1 publications with ATP5A and Muscle
 Lung Antibodies  >1 publications with ATP5A and Lung
 Kidney Antibodies  >1 publications with ATP5A and Kidney
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