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Hnrnp L (HNRNPL) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435126

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
hnRNP L (HNRNPL), Monoclonal Antibody
Full Product Name
hnRNP L (393) Mouse mAb
Product Gene Name
anti-HNRNPL antibody
Product Synonym Gene Name
hnRNP-L; HNRNPL; HNRPL; P/OKcl.14
Product Synonym Names
Heterogeneous nuclear ribonucleoprotein L; hnRNP L; HNRNPL; HNRPL
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 64kDa
3D Structure
ModBase 3D Structure for P14866
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human HNRPL.

Product Categories/Family for anti-HNRNPL antibody

Cell Marker; Subcellular Marker; Nucleoplasma
Applications Tested/Suitable for anti-HNRNPL antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-HNRNPL antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-HNRNPL antibody

All lanes : HNRPL Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 64 KDa Observed band size : 64 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-HNRNPL Monoclonal Antibody – Cat# MBS435126

Immunoprecipitation (IP) of anti-HNRNPL antibody

HNRPL was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25226 of 10 ug. Western blot was performed from the immunoprecipitate using M25226 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-HNRNPL Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435126

Flow Cytometry (FC/FACS) of anti-HNRNPL antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling HNRPL with M25226 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-HNRNPL Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435126

Other Notes

Small volumes of anti-HNRNPL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNRNPL purchase

MBS435126 is an antibody targeting HNRNPL. Also known as hnRNP L (393) Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HNRNPL. It may not necessarily be applicable to this product.

NCBI GI #
52632383
NCBI GeneID
3191
NCBI Accession #
NP_001524.2
UniProt Primary Accession #
P14866
UniProt Secondary Accession #
Q9H3P3; A6ND69; A6NIT8
UniProt Related Accession #
P14866
GenBank Nucleotide #
NM_001533.2
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein L isoform a
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein L
NCBI Official Symbol
HNRNPL
NCBI Official Synonym Symbols
HNRPL; hnRNP-L; P/OKcl.14
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein L
Protein Family
Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name
HNRNPL
UniProt Synonym Gene Names
HNRPL; hnRNP L
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein L

UniProt Comments for HNRNPL

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements (PubMed:11809897, PubMed:22570490, PubMed:24164894, PubMed:25623890, PubMed:26051023). Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts (PubMed:2687284). Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter (PubMed:11809897).
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Research Articles on HNRNPL

1. High expression of HnRNP L is associated with oral squamous cell carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HNRNPL antibody

 Reference Product  PubMed Publications
 HNRNPM antibody  >2 publications with HNRNPL and HNRNPM
 HNRNPK antibody  >2 publications with HNRNPL and HNRNPK
 HNRNPC antibody  >1 publications with HNRNPL and HNRNPC
 HNRNPA2B1 antibody  >1 publications with HNRNPL and HNRNPA2B1

Pathways associated with anti-HNRNPL antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram

Diseases associated with anti-HNRNPL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with HNRNPL and Nervous System Diseases
 Inflammation Antibodies  >2 publications with HNRNPL and Inflammation
 Necrosis Antibodies  >2 publications with HNRNPL and Necrosis
 Spinal Cord Diseases Antibodies  >1 publications with HNRNPL and Spinal Cord Diseases
 Breast Neoplasms Antibodies  >1 publications with HNRNPL and Breast Neoplasms

Organs/Tissues associated with anti-HNRNPL antibody

 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >2 publications with HNRNPL and Thymus
 Vascular Antibodies  >1 publications with HNRNPL and Vascular
 Lymph Antibodies  >1 publications with HNRNPL and Lymph
 Lymph Node Antibodies  >1 publications with HNRNPL and Lymph Node
 Lung Antibodies  >1 publications with HNRNPL and Lung
 Embryonic Tissue Antibodies  >1 publications with HNRNPL and Embryonic Tissue
 Brain Antibodies  >1 publications with HNRNPL and Brain
 Testis Antibodies  >1 publications with HNRNPL and Testis
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