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RBM17/SPF45 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435138

This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RBM17/SPF45, Monoclonal Antibody
Full Product Name
RBM17/SPF45 (881) Mouse mAb
Product Gene Name
Product Synonym Gene Name
RBM17; MGC14439; 2700027J02Rik; SPF45
Product Synonym Names
RBM17/SPF45(881) Mouse mAb; 45 kDa-splicing factor; RNA-binding motif protein 17; SPF45; Splicing factor 45; splicing factor 45kDa
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 45kDa
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human RBM17/SPF45.
Applications Tested/Suitable for anti-RBM17/SPF45 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-RBM17/SPF45 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Flow Cytometry (FC/FACS) of anti-RBM17/SPF45 antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SPF45 with M25213 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-RBM17/SPF45 Monoclonal Antibody – Cat# MBS435138

Other Notes

Small volumes of anti-RBM17/SPF45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBM17/SPF45 purchase

MBS435138 is an antibody targeting RBM17/SPF45. Also known as RBM17/SPF45 (881) Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RBM17/SPF45. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
RBM17 protein
NCBI Official Synonym Full Names
RNA binding motif protein 17
NCBI Official Symbol
RBM17
NCBI Official Synonym Symbols
SPF45
UniProt Protein Name
Splicing factor 45
UniProt Synonym Protein Names
45 kDa-splicing factor; RNA-binding motif protein 17
UniProt Gene Name
RBM17
UniProt Synonym Gene Names
SPF45
NCBI Protein Information
splicing factor 45

UniProt Comments for RBM17/SPF45

Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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