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PABP2/ PABPN1 (PABP2/PABPN1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435140

This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
PABP2/ PABPN1 (PABP2/PABPN1), Monoclonal Antibody
Full Product Name
PABP2/ PABPN1 Mouse mAb
Product Gene Name
anti-PABP2/PABPN1 antibody
Product Synonym Gene Name
mPABII; OPMD; PAB2; PABII; PABP-2; PABP2; Pabp3; PABPN1
Product Synonym Names
PABP2 Mouse mAb; Nuclear poly(A)-binding protein 1; OPMD; PAB2; PABII; PABP-2; PABP2; poly(A) binding protein 2; poly(A) binding protein II; poly(A) binding protein, nuclear 1; poly(A)-binding protein 2; Poly(A)-binding protein II; Polyadenylate-binding nuclear protein 1; Polyadenylate-binding protein 2
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 33kDa
3D Structure
ModBase 3D Structure for Q86U42
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human PABP2.

Product Categories/Family for anti-PABP2/PABPN1 antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-PABP2/PABPN1 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-PABP2/PABPN1 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-PABP2/PABPN1 antibody

All lanes : PABP2 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 33 KDa Observed band size : 50 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-PABP2/PABPN1 Monoclonal Antibody – Cat# MBS435140

Immunoprecipitation (IP) of anti-PABP2/PABPN1 antibody

PABP2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25211 of 10 ug. Western blot was performed from the immunoprecipitate using M25211 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-PABP2/PABPN1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435140

Flow Cytometry (FC/FACS) of anti-PABP2/PABPN1 antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PABP2 with M25211 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-PABP2/PABPN1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435140

Other Notes

Small volumes of anti-PABP2/PABPN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PABP2/PABPN1 purchase

MBS435140 is an antibody targeting PABP2/PABPN1. Also known as PABP2/ PABPN1 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PABP2/PABPN1. It may not necessarily be applicable to this product.

NCBI GI #
1339869160
NCBI GeneID
8106
NCBI Accession #
NP_001347480.1
UniProt Primary Accession #
Q86U42
UniProt Secondary Accession #
O43484; D3DS49
UniProt Related Accession #
Q86U42
GenBank Nucleotide #
NM_001360551.1
NCBI Official Full Name
polyadenylate-binding protein 2 isoform 2
NCBI Official Synonym Full Names
poly(A) binding protein nuclear 1
NCBI Official Symbol
PABPN1
NCBI Official Synonym Symbols
OPMD; PAB2; PABII; PABP2; PABP-2
UniProt Protein Name
Polyadenylate-binding protein 2
UniProt Synonym Protein Names
Nuclear poly(A)-binding protein 1; Poly(A)-binding protein II; PABII; Polyadenylate-binding nuclear protein 1
UniProt Gene Name
PABPN1
UniProt Synonym Gene Names
PAB2; PABP2; PABP-2; Poly(A)-binding protein 2; PABII
NCBI Protein Information
polyadenylate-binding protein 2

UniProt Comments for PABP2/PABPN1

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (). Increases the affinity of poly(A) polymerase for RNA (). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (). May protect the poly(A) tail from degradation (). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).
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Research Articles on PABP2/PABPN1

1. The expression of Ala-expanded-PABPN1 causes the formation of nuclear aggregates before the onset of muscle weakness in oculopharyngeal muscular dystrophy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PABP2/PABPN1 antibody

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