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Lamin A/C (LMNA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435142

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled… More details

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Product Overview

Online Datasheet in PDF
Product Name
Lamin A/C (LMNA), Monoclonal Antibody
Full Product Name
Lamin A/C Mouse mAb
Product Gene Name
anti-LMNA antibody
Product Synonym Gene Name
BOS_2775; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1
Product Synonym Names
Lamin A/C (LMNA) Mouse mAb; 70 kDa lamin; lamin A/C-like 1; lamin-A/C; Prelamin-A/C; renal carcinoma antigen NY-REN-32
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 65kDa
3D Structure
ModBase 3D Structure for P02545
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human LMNA.

Product Categories/Family for anti-LMNA antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-LMNA antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-LMNA antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-LMNA antibody

All lanes : LMNA Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 65 KDa Observed band size : 65 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-LMNA Monoclonal Antibody – Cat# MBS435142

Immunoprecipitation (IP) of anti-LMNA antibody

LMNA was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25209 of 10 ug. Western blot was performed from the immunoprecipitate using M25209 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-LMNA Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435142

Flow Cytometry (FC/FACS) of anti-LMNA antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling LMNA with M25209 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-LMNA Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435142

Other Notes

Small volumes of anti-LMNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LMNA purchase

MBS435142 is an antibody targeting LMNA. Also known as Lamin A/C Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LMNA. It may not necessarily be applicable to this product.

NCBI GI #
27436946
NCBI GeneID
4000
NCBI Accession #
NP_733821.1
UniProt Primary Accession #
P02545
UniProt Secondary Accession #
P02546; Q5I6Y4; Q5I6Y6; Q5TCJ2; Q5TCJ3; Q6UYC3; Q969I8; B4DI32; D3DVB0; D6RAQ3; E7EUI9
UniProt Related Accession #
P02545
GenBank Nucleotide #
NM_170707.3
NCBI Official Full Name
lamin isoform A
NCBI Official Synonym Full Names
lamin A/C
NCBI Official Symbol
LMNA
NCBI Official Synonym Symbols
FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
UniProt Protein Name
Prelamin-A/C
UniProt Synonym Protein Names
Lamin-A/CAlternative name(s):70 kDa lamin; Renal carcinoma antigen NY-REN-32
Protein Family
Lamin
UniProt Gene Name
LMNA
UniProt Synonym Gene Names
LMN1
NCBI Protein Information
lamin

UniProt Comments for LMNA

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:22431096, PubMed:10814726, PubMed:11799477, PubMed:18551513). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920).
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Research Articles on LMNA

1. Three heterozygous missense mutations were identified in unrelated patients - p. W520R (c.1558T > C), p.T528R (small es, Cyrillic.1583capital ES, Cyrillic > G) and p.R190P (c.569G > C). We consider these variants as pathogenic, leading to isolated DCM with conduction defects or syndromic DCM forms with limb-girdle muscular dystrophy and Emery- Dreifuss muscular dystrophy.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LMNA antibody

 Reference Product  PubMed Publications
 EMD antibody  >17 publications with LMNA and EMD
 LMNB1 antibody  >14 publications with LMNA and LMNB1
 LMNB2 antibody  >10 publications with LMNA and LMNB2
 SYNE1 antibody  >5 publications with LMNA and SYNE1
 CASP6 antibody  >3 publications with LMNA and CASP6
 TMPO antibody  >2 publications with LMNA and TMPO
 FOS antibody  >2 publications with LMNA and FOS

Pathways associated with anti-LMNA antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Breakdown Of The Nuclear Lamina Pathway antibodies  Breakdown Of The Nuclear Lamina Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram

Diseases associated with anti-LMNA antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >263 publications with LMNA and Cardiovascular Diseases
 Progeria Antibodies  >245 publications with LMNA and Progeria
 Heart Diseases Antibodies  >230 publications with LMNA and Heart Diseases
 Cardiomyopathy, Dilated Antibodies  >190 publications with LMNA and Cardiomyopathy, Dilated
 Muscular Dystrophy, Emery-Dreifuss Antibodies  >135 publications with LMNA and Muscular Dystrophy, Emery-Dreifuss
 Lipodystrophy, Familial Partial Antibodies  >129 publications with LMNA and Lipodystrophy, Familial Partial
 Death, Sudden, Cardiac Antibodies  >47 publications with LMNA and Death, Sudden, Cardiac
 Muscular Dystrophies, Limb-Girdle Antibodies  >38 publications with LMNA and Muscular Dystrophies, Limb-Girdle
 Heart Block Antibodies  >32 publications with LMNA and Heart Block
 Werner Syndrome Antibodies  >27 publications with LMNA and Werner Syndrome

Organs/Tissues associated with anti-LMNA antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >261 publications with LMNA and Muscle
 Heart Antibodies  >170 publications with LMNA and Heart
 Skin Antibodies  >114 publications with LMNA and Skin
 Adipose Tissue Antibodies  >87 publications with LMNA and Adipose Tissue
 Blood Antibodies  >78 publications with LMNA and Blood
 Bone Antibodies  >70 publications with LMNA and Bone
 Nerve Antibodies  >50 publications with LMNA and Nerve
 Connective Tissue Antibodies  >44 publications with LMNA and Connective Tissue
 Vascular Antibodies  >40 publications with LMNA and Vascular
 Brain Antibodies  >23 publications with LMNA and Brain
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