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KRT6B/K2C6B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435143

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified… More details

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Product Overview

Online Datasheet in PDF
Product Name
KRT6B/K2C6B, Monoclonal Antibody
Full Product Name
KRT6B/K2C6B Mouse mAb
Product Gene Name
anti-KRT6B/K2C6B antibody
Product Synonym Gene Name
BB144589; CK-6B; CK6B; K6B; Krt2-6b; KRT6B; KRTL1; mK6[b]; PC2; PC4
Product Synonym Names
CK-6B; CK6B; cytokeratin 6B; Cytokeratin-6B; epidermal; K6B; keratin; keratin 6B, type II; keratin complex 2, basic, gene 6b; keratin, epidermal, type II, K6B; Keratin, type II cytoskeletal 6B; keratin-6-beta; Keratin-6B; keratin-like 1 (a type II keratin sequence); KRTL1; mK6-beta; type II; type II cytoskeletal 6B; Type-II keratin Kb10
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 60kDa
3D Structure
ModBase 3D Structure for P04259
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human KRT6B/K2C6B.

Product Categories/Family for anti-KRT6B/K2C6B antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-KRT6B/K2C6B antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-KRT6B/K2C6B antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-KRT6B/K2C6B antibody

All lanes : K2C6B Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-KRT6B/K2C6B Monoclonal Antibody – Cat# MBS435143

Immunoprecipitation (IP) of anti-KRT6B/K2C6B antibody

K2C6B was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25207 of 10 ug. Western blot was performed from the immunoprecipitate using M25207 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-KRT6B/K2C6B Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435143

Flow Cytometry (FC/FACS) of anti-KRT6B/K2C6B antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling K2C6B with M25207 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-KRT6B/K2C6B Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435143

Other Notes

Small volumes of anti-KRT6B/K2C6B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT6B/K2C6B purchase

MBS435143 is an antibody targeting KRT6B/K2C6B. Also known as KRT6B/K2C6B Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT6B/K2C6B. It may not necessarily be applicable to this product.

NCBI GI #
119703753
NCBI GeneID
3854
NCBI Accession #
NP_005546.2
UniProt Primary Accession #
P04259
UniProt Secondary Accession #
P48669
UniProt Related Accession #
P04259
GenBank Nucleotide #
NM_005555.3
NCBI Official Full Name
keratin, type II cytoskeletal 6B
NCBI Official Synonym Full Names
keratin 6B
NCBI Official Symbol
KRT6B
NCBI Official Synonym Symbols
K6B; PC2; PC4; CK6B; CK-6B; KRTL1
UniProt Protein Name
Keratin, type II cytoskeletal 6B
UniProt Synonym Protein Names
Cytokeratin-6B; CK-6B; Keratin-6B; K6B; Type-II keratin Kb10
UniProt Gene Name
KRT6B
UniProt Synonym Gene Names
K6B; KRTL1; CK-6B; K6B
NCBI Protein Information
keratin, type II cytoskeletal 6B

NCBI Summary for KRT6B/K2C6B

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT6B/K2C6B

MiscellaneousThere are at least six isoforms of human type II keratin-6 (K6).

Research Articles on KRT6B/K2C6B

1. Data indicate that cytokeratin 5/6 (CK5/6) is an independent prognostic biomarker in urothelial carcinoma and therefore can be used in the prognostic stratification of the patients with bladder cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT6B/K2C6B antibody

 Reference Product  PubMed Publications
 KRT6A antibody  >33 publications with KRT6B/K2C6B and KRT6A
 KRT16 antibody  >19 publications with KRT6B/K2C6B and KRT16
 KRT17 antibody  >17 publications with KRT6B/K2C6B and KRT17
 KRT5 antibody  >12 publications with KRT6B/K2C6B and KRT5
 SPRR1A antibody  >1 publications with KRT6B/K2C6B and SPRR1A

Diseases associated with anti-KRT6B/K2C6B antibody

 Disease Name  Pubmed Publications
 Pachyonychia Congenita Antibodies  >18 publications with KRT6B/K2C6B and Pachyonychia Congenita
 Congenital Abnormalities Antibodies  >12 publications with KRT6B/K2C6B and Congenital Abnormalities
 Breast Neoplasms Antibodies  >7 publications with KRT6B/K2C6B and Breast Neoplasms
 Lung Neoplasms Antibodies  >5 publications with KRT6B/K2C6B and Lung Neoplasms
 Adenocarcinoma Antibodies  >4 publications with KRT6B/K2C6B and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >4 publications with KRT6B/K2C6B and Carcinoma, Squamous Cell
 Inflammation Antibodies  >2 publications with KRT6B/K2C6B and Inflammation
 Neoplasms, Experimental Antibodies  >1 publications with KRT6B/K2C6B and Neoplasms, Experimental
 Necrosis Antibodies  >1 publications with KRT6B/K2C6B and Necrosis
 Hyperplasia Antibodies  >1 publications with KRT6B/K2C6B and Hyperplasia

Organs/Tissues associated with anti-KRT6B/K2C6B antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >9 publications with KRT6B/K2C6B and Skin
 Lung Antibodies  >5 publications with KRT6B/K2C6B and Lung
 Mouth Antibodies  >4 publications with KRT6B/K2C6B and Mouth
 Muscle Antibodies  >1 publications with KRT6B/K2C6B and Muscle
 Thyroid Antibodies  >1 publications with KRT6B/K2C6B and Thyroid
 Brain Antibodies  >1 publications with KRT6B/K2C6B and Brain
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