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FAM62A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435145

Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to… More details

$435 / 0.05 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FAM62A, Monoclonal Antibody
Full Product Name
FAM62A Mouse mAb
Product Gene Name
Product Synonym Gene Name
KIAA0747; MBC2
Product Synonym Names
family with sequence similarity62 (C2 domain containing), member A; KIAA0747, membrane bound C2 domain containing protein; vp115; MBC2; FAM62A; family with sequence similarity 62 (C2 domain containing), member A
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 124kDa
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of FAM62A.
Applications Tested/Suitable for anti-FAM62A antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-FAM62A antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-FAM62A antibody

All lanes : ESYT1 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 124 KDa Observed band size : 124 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-FAM62A Monoclonal Antibody – Cat# MBS435145

Immunoprecipitation (IP) of anti-FAM62A antibody

ESYT1 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25205 of 10 ug. Western blot was performed from the immunoprecipitate using M25205 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-FAM62A Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435145

Flow Cytometry (FC/FACS) of anti-FAM62A antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling ESYT1 with M25205 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-FAM62A Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435145

Other Notes

Small volumes of anti-FAM62A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAM62A purchase

MBS435145 is an antibody targeting FAM62A. Also known as FAM62A Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FAM62A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
family with sequence similarity 62 (C2 domain containing), member A, isoform CRA_a
NCBI Official Synonym Full Names
extended synaptotagmin 1
NCBI Official Symbol
ESYT1
NCBI Official Synonym Symbols
MBC2; FAM62A
UniProt Protein Name
Extended synaptotagmin-1
UniProt Synonym Protein Names
Membrane-bound C2 domain-containing protein
UniProt Gene Name
ESYT1
UniProt Synonym Gene Names
FAM62A; KIAA0747; MBC2; E-Syt1
NCBI Protein Information
extended synaptotagmin-1

UniProt Comments for FAM62A

Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-FAM62A antibody

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