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ADP/ATP Translocase 2 (SLC25A5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435147

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into… More details

$435 / 0.05 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ADP/ATP translocase 2 (SLC25A5), Monoclonal Antibody
Full Product Name
ADP/ATP translocase 2 (458) Mouse mAb
Product Gene Name
anti-SLC25A5 antibody
Product Synonym Names
Adenine nucleotide translocator 2; adenine nucleotide translocator 2 (fibroblast); ADP, ATP carrier protein 2; ADP, ATP carrier protein, fibroblast isoform; ADP/ATP translocase 2; ANT 2; ANT2; ATP carrier protein; ATP carrier protein 2; DP/ATP translocase 2; fibroblast isoform; N-terminally processed; solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5; Solute carrier family 25 member 5; 2F1, ANT2, T2, T3
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20°C. Stable for one year from the date of receipt.
Molecular Weight
Predicted MW: 33kDa
3D Structure
ModBase 3D Structure for P05141
Source Note
This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to ADT2 protein.

Product Categories/Family for anti-SLC25A5 antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-SLC25A5 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC)
Application Notes for anti-SLC25A5 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC: 1:100-1:200
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.05% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-SLC25A5 antibody

All lanes : ADT2 Mouse mAb at 1/2000 dilution
Lane 1 : THP1 cells membrane fraction
Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution
Predicted band size : 33 KDa
Observed band size : 33 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-SLC25A5 Monoclonal Antibody – Cat# MBS435147

Immunoprecipitation (IP) of anti-SLC25A5 antibody

ADT2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with MBS435147 of 10 ug. Western blot was performed from the immunoprecipitate using MBS435147 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.
Lane 1: THP1 cells membrane fraction.
Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration: 5% milk/TBST.
Mouse Anti-SLC25A5 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435147

Flow Cytometry (FC) of anti-SLC25A5 antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling ADT2 with M25202 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-SLC25A5 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS435147

Other Notes

Small volumes of anti-SLC25A5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC25A5 purchase

MBS435147 is an antibody targeting SLC25A5. Also known as ADP/ATP translocase 2 (458) Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC25A5. It may not necessarily be applicable to this product.

NCBI GI #
156071459
NCBI GeneID
292
NCBI Accession #
NP_001143.2
UniProt Primary Accession #
P05141
UniProt Secondary Accession #
O43350; B2RCV1
UniProt Related Accession #
P05141
GenBank Nucleotide #
NM_001152.4
NCBI Official Full Name
ADP/ATP translocase 2
NCBI Official Synonym Full Names
solute carrier family 25 member 5
NCBI Official Symbol
SLC25A5
NCBI Official Synonym Symbols
T2; T3; 2F1; AAC2; ANT2
UniProt Protein Name
ADP/ATP translocase 2
UniProt Synonym Protein Names
ADP,ATP carrier protein 2; ADP,ATP carrier protein, fibroblast isoform; Adenine nucleotide translocator 2; ANT 2
Protein Family
ADP/ATP translocase
UniProt Gene Name
SLC25A5
UniProt Synonym Gene Names
ANT2; ANT 2
NCBI Protein Information
ADP/ATP translocase 2

UniProt Comments for SLC25A5

Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.

Research Articles on SLC25A5

1. Findings suggest that ANT2 overexpression contributes to EGFR-TKI resistance in non-small cell lung cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC25A5 antibody

 Reference Product  PubMed Publications
 VDAC3 antibody  >1 publications with SLC25A5 and VDAC3

Pathways associated with anti-SLC25A5 antibody

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Host Interactions Of HIV Factors Pathway antibodies  Host Interactions Of HIV Factors Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram

Diseases associated with anti-SLC25A5 antibody

 Disease Name  Pubmed Publications
 Neurobehavioral Manifestations Antibodies  >3 publications with SLC25A5 and Neurobehavioral Manifestations
 Anemia Antibodies  >1 publications with SLC25A5 and Anemia
 Liver Diseases Antibodies  >1 publications with SLC25A5 and Liver Diseases
 Hepatomegaly Antibodies  >1 publications with SLC25A5 and Hepatomegaly
 Carcinoma, Hepatocellular Antibodies  >1 publications with SLC25A5 and Carcinoma, Hepatocellular
 Infertility, Male Antibodies  >1 publications with SLC25A5 and Infertility, Male
 Liver Neoplasms Antibodies  >1 publications with SLC25A5 and Liver Neoplasms

Organs/Tissues associated with anti-SLC25A5 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with SLC25A5 and Liver
 Skin Antibodies  >3 publications with SLC25A5 and Skin
 Brain Antibodies  >3 publications with SLC25A5 and Brain
 Embryonic Tissue Antibodies  >2 publications with SLC25A5 and Embryonic Tissue
 Heart Antibodies  >2 publications with SLC25A5 and Heart
 Ovary Antibodies  >1 publications with SLC25A5 and Ovary
 Testis Antibodies  >1 publications with SLC25A5 and Testis
 Stomach Antibodies  >1 publications with SLC25A5 and Stomach
 Eye Antibodies  >1 publications with SLC25A5 and Eye
 Kidney Antibodies  >1 publications with SLC25A5 and Kidney
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