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P5CS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435154

This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP-and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction… More details

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Product Overview

Online Datasheet in PDF
Product Name
P5CS, Monoclonal Antibody
Full Product Name
P5CS (012) Mouse mAb
Product Gene Name
anti-P5CS antibody
Product Synonym Names
P5CS(012) Mouse mAb; aldehyde dehydrogenase 18 family, member A1; aldehyde dehydrogenase 18A1; Aldehyde dehydrogenase family 18 member A1; ALDH18A1; Delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; delta1-pyrroline-5-carboxlate synthetase; glutamate gamma-semialdehyde synthetase; GSAS; MGC117316; P5CS; PYCS; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase); 2810433K04Rik; ADCL3; AI429789; ALDH18A1; ARCL3A; GSAS; P5CS; PYCS; SPG9A; SPG9B
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Homo sapiens
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 87kDa
3D Structure
ModBase 3D Structure for P54886
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human P5CS

Product Categories/Family for anti-P5CS antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-P5CS antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-P5CS antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-P5CS antibody

Mouse Anti-P5CS Monoclonal Antibody – Cat# MBS435154

Immunoprecipitation (IP) of anti-P5CS antibody

P5CS was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with MBS435154 of 10 ug. Western blot was performed from the immunoprecipitate using M25111 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 87 KDa
Observed band size : 87 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-P5CS Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435154

Flow Cytometry (FC/FACS) of anti-P5CS antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling P5CS with M25111 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-P5CS Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435154

Other Notes

Small volumes of anti-P5CS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for P5CS purchase

MBS435154 is an antibody targeting P5CS. Also known as P5CS (012) Mouse mAb. Reactive in Homo sapiens. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for P5CS. It may not necessarily be applicable to this product.

NCBI GI #
62912457
NCBI GeneID
5832
NCBI Accession #
NP_001017423.1
UniProt Primary Accession #
P54886
UniProt Secondary Accession #
O95952; Q3KQU2; Q5T566; Q5T567; Q9UM72; B2R5Q4; B7Z350; B7Z5X8; B7ZLP1; D3DR44
UniProt Related Accession #
P54886
GenBank Nucleotide #
NM_001017423.1
NCBI Official Full Name
delta-1-pyrroline-5-carboxylate synthase isoform 2
NCBI Official Synonym Full Names
aldehyde dehydrogenase 18 family member A1
NCBI Official Symbol
ALDH18A1
NCBI Official Synonym Symbols
GSAS; P5CS; PYCS; SPG9; ADCL3; SPG9A; SPG9B; ARCL3A
UniProt Protein Name
Delta-1-pyrroline-5-carboxylate synthase
UniProt Synonym Protein Names
Aldehyde dehydrogenase family 18 member A1Including the following 2 domains:Glutamate 5-kinase (EC:2.7.2.111 PublicationManual assertion based on experiment iniRef.14"ALDH18A1 gene mutations cause dominant spastic paraplegia SPG9: loss of function effect and plausibility of a dominant negative mechanism."Panza E., Escamilla-Honrubia J.M., Marco-Marin C., Gougeard N., De Michele G., Morra V.B., Liguori R., Salviati L., Donati M.A., Cusano R., Pippucci T., Ravazzolo R., Nemeth A.H., Smithson S., Davies S., Hurst J.A., Bordo D., Rubio V., Seri M.Brain 139:E3-E3(2016) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBUNIT, SUBCELLULAR LOCATION, INVOLVEMENT IN SPG9A, VARIANTS SPG9A LEU-243 AND GLN-252, CHARACTERIZATION OF VARIANTS SPG9A LEU-243 AND GLN-252.
Protein Family
Delta-1-pyrroline-5-carboxylate synthase
UniProt Gene Name
ALDH18A1
UniProt Synonym Gene Names
GSAS; P5CS; PYCS; P5CS; GK; GPR
NCBI Protein Information
delta-1-pyrroline-5-carboxylate synthase

NCBI Summary for P5CS

This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for P5CS

Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.

Research Articles on P5CS

1. This is the first report of an individual with ALDH18A1-ADCL due to a substitution at a residue other than p.Arg138. Knowledge of the complete spectrum of dominant-acting mutations that cause this rare syndrome will have implications for molecular diagnosis and genetic counselling of these families.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-P5CS antibody

 Products by Pathway  Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Proline Biosynthesis, Glutamate => Proline Pathway antibodies  Proline Biosynthesis, Glutamate => Proline Pathway Diagram
 Proline Biosynthesis, Glutamate => Proline Pathway antibodies  Proline Biosynthesis, Glutamate => Proline Pathway Diagram

Diseases associated with anti-P5CS antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with P5CS and Nervous System Diseases
 Congenital Abnormalities Antibodies  >3 publications with P5CS and Congenital Abnormalities
 Muscular Diseases Antibodies  >1 publications with P5CS and Muscular Diseases
 Heart Defects, Congenital Antibodies  >1 publications with P5CS and Heart Defects, Congenital

Organs/Tissues associated with anti-P5CS antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with P5CS and Skin
 Vascular Antibodies  >1 publications with P5CS and Vascular
 Eye Antibodies  >1 publications with P5CS and Eye
 Connective Tissue Antibodies  >1 publications with P5CS and Connective Tissue
 Blood Antibodies  >1 publications with P5CS and Blood
 Heart Antibodies  >1 publications with P5CS and Heart
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