• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Vimentin (VIM) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435155

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Monoclonal Antibody
Full Product Name
Vimentin (214) Mouse mAb
Product Gene Name
anti-VIM antibody
Product Synonym Gene Name
VIME
Product Synonym Names
epididymis luminal protein 113; FLJ36605; Vimentin; CTRCT30; HEL113
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 54kDa
3D Structure
ModBase 3D Structure for P08670
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human VIME

Product Categories/Family for anti-VIM antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-VIM antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-VIM antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-VIM antibody

Mouse Anti-VIM Monoclonal Antibody – Cat# MBS435155

Immunoprecipitation (IP) of anti-VIM antibody

VIME was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25110 of 10 ug. Western blot was performed from the immunoprecipitate using M25110 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 54 KDa
Observed band size : 54 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-VIM Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435155

Flow Cytometry (FC/FACS) of anti-VIM antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling VIME with M25110 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-VIM Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435155

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS435155 is an antibody targeting VIM. Also known as Vimentin (214) Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.4
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Show more

UniProt Comments for VIM

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Research Articles on VIM

1. the HDAC inhibitors augmented both Ecadherin and vimentin expression and their effects varied in different cholangiocarcinoma cell lines. Therefore, the clinical use of HDAC inhibitors in biliary cancer should be considered cautiously

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >383 publications with VIM and Nervous System Diseases
 Neoplasms Antibodies  >283 publications with VIM and Neoplasms
 Brain Diseases Antibodies  >246 publications with VIM and Brain Diseases
 Neurodegenerative Diseases Antibodies  >166 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >46 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >37 publications with VIM and Kidney Diseases
 Hemorrhage Antibodies  >32 publications with VIM and Hemorrhage
 Disease Models, Animal Antibodies  >32 publications with VIM and Disease Models, Animal
 Pain Antibodies  >31 publications with VIM and Pain
 Liver Diseases Antibodies  >29 publications with VIM and Liver Diseases

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >461 publications with VIM and Brain
 Blood Antibodies  >187 publications with VIM and Blood
 Muscle Antibodies  >101 publications with VIM and Muscle
 Nerve Antibodies  >79 publications with VIM and Nerve
 Bone Antibodies  >69 publications with VIM and Bone
 Kidney Antibodies  >52 publications with VIM and Kidney
 Liver Antibodies  >51 publications with VIM and Liver
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Eye Antibodies  >38 publications with VIM and Eye
 Vascular Antibodies  >35 publications with VIM and Vascular
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000