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MIMIT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435162

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane.… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MIMIT, Monoclonal Antibody
Full Product Name
MIMIT Mouse mAb
Product Gene Name
Product Synonym Names
Mimitin /NDUFAF2 Mouse mAb; B17.2-like; B17.2L; B172-like; B172L; Mimitin; Mimitin, mitochondrial; mitochondrial; MMTN; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; NADH dehydrogenase (ubiquinone) complex I, assembly factor 2; NADH dehydrogenase 1 alpha subcomplex assembly factor 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein; NDUFA12L; NDUFAF2; B17.2L; mimitin; MMTN; NDUFA12L; NDUFAF2
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 20kDa
3D Structure
ModBase 3D Structure for Q8N183
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human MIMIT
Applications Tested/Suitable for anti-MIMIT antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-MIMIT antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-MIMIT antibody

Mouse Anti-MIMIT Monoclonal Antibody – Cat# MBS435162

Immunoprecipitation (IP) of anti-MIMIT antibody

MIMIT was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25103 of 10 ug. Western blot was performed from the immunopre-cipitate using M25103 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 20 KDa
Observed band size : 20 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-MIMIT Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435162

Flow Cytometry (FC/FACS) of anti-MIMIT antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MIMIT with M25103 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-MIMIT Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435162

Other Notes

Small volumes of anti-MIMIT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIMIT purchase

MBS435162 is an antibody targeting MIMIT. Also known as MIMIT Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MIMIT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase complex assembly factor 2
NCBI Official Symbol
NDUFAF2
NCBI Official Synonym Symbols
MMTN; B17.2L; mimitin; NDUFA12L
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2
UniProt Synonym Protein Names
B17.2-like; B17.2L; Mimitin
Protein Family
UniProt Gene Name
NDUFAF2
UniProt Synonym Gene Names
NDUFA12L; MMTN1 PublicationManual assertion based on opinion iniRef.1
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2

UniProt Comments for MIMIT

Acts as a molecular chaperone for mitochondrial complex I assembly (PubMed:16200211). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:16200211, PubMed:27626371).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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