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MAD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435163

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAD1, Monoclonal Antibody
Full Product Name
MAD1 Mouse mAb
Product Gene Name
Product Synonym Gene Name
MAD1; PIG9; TP53I9; TXBP181
Product Synonym Names
homolog-like 1; HsMAD1; MAD1 mitotic arrest deficient-like 1; MAD1-like protein 1; mitotic arrest deficient 1-like 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; mitotic-arrest deficient 1; mitotic-arrest deficient 1, yeast, homolog-like 1; PIG9; Tax-binding protein 181; TP53I9; tumor protein p53 inducible pr; tumor protein p53 inducible protein 9; TXBP181; yeast; AI173502; AW550425; MAD1; MAD1L1; PIG9; TP53I9; TXBP181
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 83kDa
3D Structure
ModBase 3D Structure for Q9Y6D9
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human MD1L1
Applications Tested/Suitable for anti-MAD1 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-MAD1 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-MAD1 antibody

Mouse Anti-MAD1 Monoclonal Antibody – Cat# MBS435163

Immunoprecipitation (IP) of anti-MAD1 antibody

MD1L1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25102 of 10 ug. Western blot was performed from the immunopre-cipitate using M25102 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 83 KDa
Observed band size : 83 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-MAD1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435163

Flow Cytometry (FC/FACS) of anti-MAD1 antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MD1L1 with M25102 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-MAD1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435163

Other Notes

Small volumes of anti-MAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAD1 purchase

MBS435163 is an antibody targeting MAD1. Also known as MAD1 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD1 isoform a
NCBI Official Synonym Full Names
mitotic arrest deficient 1 like 1
NCBI Official Symbol
MAD1L1
NCBI Official Synonym Symbols
MAD1; PIG9; TP53I9; TXBP181
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD1
UniProt Synonym Protein Names
Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181
UniProt Gene Name
MAD1L1
UniProt Synonym Gene Names
MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD1

UniProt Comments for MAD1

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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