• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

F162A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435167

Proposed to be involved in regulation of apoptosis; the exact mechanism may differ between cell types, tissues. May be involved in hypoxia-induced cell death of transformed cells implicating cytochrome C release and caspase activation (such as CASP9) and… More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F162A, Monoclonal Antibody
Full Product Name
F162A Mouse mAb
Product Gene Name
Product Synonym Names
C3orf28; E2-induced gene 5 protein; E2-induced gene 5 protein homolog; E2IG5; family with sequence similarity 162, member A; Growth and transformation-dependent protein; HGTD-P; HIF-1 alpha-responsive proapoptotic molecule; Protein FAM162A; 2310056P07Rik; C3orf28; DC16; E2IG5; FAM162A; FWP001; HGTD-P
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 17kDa
3D Structure
ModBase 3D Structure for Q96A26
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human F162A
Applications Tested/Suitable for anti-F162A antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-F162A antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-F162A antibody

Mouse Anti-F162A Monoclonal Antibody – Cat# MBS435167

Immunoprecipitation (IP) of anti-F162A antibody

F162A was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25098 of 10 ug. Western blot was performed from the immunopre-cipitate using M25098 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Predicted band size : 17 KDa
Observed band size : 17 KDa

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-F162A Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435167

Flow Cytometry (FC/FACS) of anti-F162A antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling F162A with M25098 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-F162A Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435167

Other Notes

Small volumes of anti-F162A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F162A purchase

MBS435167 is an antibody targeting F162A. Also known as F162A Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for F162A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein FAM162A
NCBI Official Synonym Full Names
family with sequence similarity 162 member A
NCBI Official Symbol
FAM162A
NCBI Official Synonym Symbols
E2IG5; HGTD-P; C3orf28
UniProt Protein Name
Protein FAM162A
UniProt Synonym Protein Names
E2-induced gene 5 protein; Growth and transformation-dependent protein; HGTD-P
UniProt Gene Name
FAM162A
UniProt Synonym Gene Names
C3orf28; E2IG5; HGTD-P
NCBI Protein Information
protein FAM162A

UniProt Comments for F162A

Proposed to be involved in regulation of apoptosis; the exact mechanism may differ between cell types/tissues. May be involved in hypoxia-induced cell death of transformed cells implicating cytochrome C release and caspase activation (such as CASP9) and inducing mitochondrial permeability transition. May be involved in hypoxia-induced cell death of neuronal cells probably by promoting release of AIFM1 from mitochondria to cytoplasm and its translocation to the nucleus; however, the involvement of caspases has been reported conflictingly.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000