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RNF40/BRE1B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435178

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that… More details

$435 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
RNF40/BRE1B, Monoclonal Antibody
Full Product Name
RNF40/BRE1B Mouse mAb
Product Gene Name
Product Synonym Names
95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; E3 ubiquitin-protein ligase BRE1B; Rb-associated protein; RBP95; RING finger protein 40; ring finger protein 40, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase BRE1B; STARING; BRE1B; KIAA0661; RBP95; RNF40; STARING
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 114kDa
3D Structure
ModBase 3D Structure for O75150
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human BRE1B
Applications Tested/Suitable for anti-RNF40/BRE1B antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-RNF40/BRE1B antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-RNF40/BRE1B antibody

Mouse Anti-RNF40/BRE1B Monoclonal Antibody – Cat# MBS435178

Flow Cytometry (FC/FACS) of anti-RNF40/BRE1B antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling BRE1B with M25085 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-RNF40/BRE1B Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435178

Other Notes

Small volumes of anti-RNF40/BRE1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNF40/BRE1B purchase

MBS435178 is an antibody targeting RNF40/BRE1B. Also known as RNF40/BRE1B Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF40/BRE1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase BRE1B isoform 2
NCBI Official Synonym Full Names
ring finger protein 40
NCBI Official Symbol
RNF40
NCBI Official Synonym Symbols
BRE1B; RBP95; STARING
UniProt Protein Name
E3 ubiquitin-protein ligase BRE1B
UniProt Synonym Protein Names
95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40; RING-type E3 ubiquitin transferase BRE1BCurated
UniProt Gene Name
RNF40
UniProt Synonym Gene Names
BRE1B; KIAA0661; BRE1-B; RBP95
NCBI Protein Information
E3 ubiquitin-protein ligase BRE1B

UniProt Comments for RNF40/BRE1B

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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