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ALG5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435185

This gene encodes a member of the glycosyltransferase 2 family. The encoded protein participates in glucosylation of the oligomannose core in N-linked glycosylation of proteins. The addition of glucose residues to the oligomannose core is necessary to ensure… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ALG5, Monoclonal Antibody
Full Product Name
ALG5 Mouse mAb
Product Gene Name
anti-ALG5 antibody
Product Synonym Names
Alg5 homolog; Alg5, S. cerevisiae, homolog of; asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphate beta-glucosyltransferase); asparagine-linked glycosylation 5 homolog (yeast, dolichyl-phosphate beta-glucosyltransferase); asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog; Asparagine-linked glycosylation protein 5 homolog; dolichyl phosphate glucosyltransferase; Dolichyl-phosphate beta-glucosyltransferase; DolP-glucosyltransferase; 1500026A19Rik; 2600005J22Rik; ALG5; AV009521; AV170001; AW545564; bA421P11.2; HSPC149
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Molecular Weight
Predicted MW: 37kDa
3D Structure
ModBase 3D Structure for Q9Y673
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human ALG5

Product Categories/Family for anti-ALG5 antibody

Cell Marker; Subcellular Marker; Membrane
Applications Tested/Suitable for anti-ALG5 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-ALG5 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-ALG5 antibody

All lanes : ALG5 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 37 KDa Observed band size : 37 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-ALG5 Monoclonal Antibody – Cat# MBS435185

Immunoprecipitation (IP) of anti-ALG5 antibody

ALG5 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25006 of 10 ug. Western blot was performed from the immunoprecipitate using M25006 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-ALG5 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435185

Flow Cytometry (FC/FACS) of anti-ALG5 antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ALG5 with M25006 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-ALG5 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435185

Other Notes

Small volumes of anti-ALG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALG5 purchase

MBS435185 is an antibody targeting ALG5. Also known as ALG5 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ALG5. It may not necessarily be applicable to this product.

NCBI GI #
215276969
NCBI GeneID
29880
NCBI Accession #
NP_001135836.1
UniProt Primary Accession #
Q9Y673
UniProt Secondary Accession #
Q5TBA6; B4DR37
UniProt Related Accession #
Q9Y673
GenBank Nucleotide #
NM_001142364.1
NCBI Official Full Name
dolichyl-phosphate beta-glucosyltransferase isoform 2
NCBI Official Synonym Full Names
ALG5, dolichyl-phosphate beta-glucosyltransferase
NCBI Official Symbol
ALG5
NCBI Official Synonym Symbols
bA421P11.2
UniProt Protein Name
Dolichyl-phosphate beta-glucosyltransferase
UniProt Synonym Protein Names
Asparagine-linked glycosylation protein 5 homolog
Protein Family
Dolichyl-phosphate beta-glucosyltransferase
UniProt Gene Name
ALG5
UniProt Synonym Gene Names
DolP-glucosyltransferase
NCBI Protein Information
dolichyl-phosphate beta-glucosyltransferase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ALG5 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies  Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Synthesis Of Dolichyl-phosphate-glucose Pathway antibodies  Synthesis Of Dolichyl-phosphate-glucose Pathway Diagram
 Synthesis Of Substrates In N-glycan Biosythesis Pathway antibodies  Synthesis Of Substrates In N-glycan Biosythesis Pathway Diagram
 Dolichyl-diphosphooligosaccharide Biosynthesis Pathway antibodies  Dolichyl-diphosphooligosaccharide Biosynthesis Pathway Diagram

Organs/Tissues associated with anti-ALG5 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >1 publications with ALG5 and Embryonic Tissue
 Skin Antibodies  >1 publications with ALG5 and Skin
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