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GP2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380003

MBS4380003 is an antibody targeting GP2. Also known as GP2 (Glycoprotein 2)/ZAP75. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GP2 (Glycoprotein 2)/ZAP75, Monoclonal Antibody
Full Product Name
GP2 (Glycoprotein 2)/ZAP75
Product Gene Name
Product Synonym Gene Name
ZAP75
Product Synonym Names
Glycoprotein 2 (zymogen granule membrane); GP2; Pancreatic zymogen granule membrane associated protein GP2; Pancreatic zymogen granule membrane protein GP-2; ZAP75
Clonality
Monoclonal
Isotype
IgG2b / Lambda
Clone Number
GP2/1712
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
59kDa
3D Structure
ModBase 3D Structure for P55259
Immunogen
Recombinant human GP2 protein fragment (aa35-179)
Cellular Localization
Apical cell membrane, Cell membrane, Endosome, Membrane raft, Secreted, Zymogen granule membrane
Positive Control
PANC-1 cells. Pancreas
Applications Tested/Suitable for anti-GP2 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-GP2 antibody
Immunohistochemistry (IHC):
1-2ug/ml 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Western Blot (WB):
2-4ug/ml

Immunohistochemistry (IHC) of anti-GP2 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with GP2 Monoclonal Antibody (GP2/1712).
Mouse Anti-GP2 Monoclonal Antibody – Cat# MBS4380003

SDS-PAGE (SDS) of anti-GP2 antibody

SDS-PAGE Analysis of Purified GP2 Mouse Monoclonal Antibody (GP2/1712). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-GP2 Monoclonal Antibody, SDS-PAGE (SDS) – Cat# MBS4380003

Testing Data (TD) of anti-GP2 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteinsusing GP2 Mouse Monoclonal Antibody (GP2/1712) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S- score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Mouse Anti-GP2 Monoclonal Antibody, Testing Data (TD) – Cat# MBS4380003

Western Blot (WB) of anti-GP2 antibody

Western Blot Analysis of human Pancreas tissue lysate using GP2 Mouse Monoclonal Antibody (GP2/1712).
Mouse Anti-GP2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380003

Testing Data (TD) of anti-GP2 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with GP2 Mouse Monoclonal Antibody (GP2/1712).
Mouse Anti-GP2 Monoclonal Antibody, Testing Data (TD) – Cat# MBS4380003

Product References and Citations for anti-GP2 antibody

Fukuoka, S., et al. 1991. A single gene encodes membrane-bound and free forms of GP-2, the major glycoprotein in pancreatic secretory (zymogen) granule membranes. Proc. Natl. Acad. Sci. USA 88: 2898-2902.

Other Notes

Small volumes of anti-GP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pancreatic secretory granule membrane major glycoprotein GP2 isoform 1 preproprotein
NCBI Official Synonym Full Names
glycoprotein 2
NCBI Official Symbol
GP2
NCBI Official Synonym Symbols
ZAP75
UniProt Protein Name
Pancreatic secretory granule membrane major glycoprotein GP2
UniProt Synonym Protein Names
Pancreatic zymogen granule membrane protein GP-2; ZAP75
UniProt Gene Name
GP2
NCBI Protein Information
pancreatic secretory granule membrane major glycoprotein GP2

NCBI Summary for GP2

This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
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UniProt Comments for GP2

CautionIt is uncertain whether Met-1 or Met-8 is the initiator.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GP2 antibody

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