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MyoD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380126

MBS4380126 is an antibody targeting MyoD1. Also known as MyoD1 (Rhabdomyosarcoma Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MyoD1, Monoclonal Antibody
Full Product Name
MyoD1 (Rhabdomyosarcoma Marker)
Product Gene Name
Product Synonym Names
bHLHc1; Class C basic helix-loop-helix protein 1; Myoblast determination protein 1; Myogenic differentiation 1; Myogenic factor 3 (Myf-3); Myogenin D1; PUM
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
rMYD712
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Specificity
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
45kDa
3D Structure
ModBase 3D Structure for P15172
Immunogen
Recombinant full-length human MyoD1 protein
Cellular Localization
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Hu-chromosome Location
11p15.1
Positive Control
Rhabdomyosarcoma
Applications Tested/Suitable for anti-MyoD1 antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-MyoD1 antibody
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-MyoD1 antibody

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712).
Mouse Anti-MyoD1 Monoclonal Antibody – Cat# MBS4380126

SDS-Page of anti-MyoD1 antibody

SDS-PAGE Analysis Purified MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712).
Mouse Anti-MyoD1 Monoclonal Antibody, SDS-Page – Cat# MBS4380126

Testing Data of anti-MyoD1 antibody

Analysis of Protein Array containing more than 19, 000 full-length human proteins using MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-MyoD1 Monoclonal Antibody, Testing Data – Cat# MBS4380126

Product References and Citations for anti-MyoD1 antibody

Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11. Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9. Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.

Other Notes

Small volumes of anti-MyoD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MyoD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myoblast determination protein 1
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
UniProt Protein Name
Myoblast determination protein 1
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
UniProt Gene Name
MYOD1
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
NCBI Protein Information
myoblast determination protein 1

NCBI Summary for MyoD1

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
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UniProt Comments for MyoD1

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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