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Neurofilament (NF-H) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380134

MBS4380134 is an antibody targeting NF-H. Also known as Neurofilament (NF-H) (Neuronal Marker). Reactive in Human, Mouse, Rat, Pig, Chicken. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Neurofilament (NF-H), Monoclonal Antibody
Full Product Name
Neurofilament (NF-H) (Neuronal Marker)
Product Gene Name
anti-NF-H antibody
Product Synonym Gene Name
NEFH
Product Synonym Names
NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200
Clonality
Monoclonal
Isotype
IgG1
Clone Number
rNF421
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Pig, Chicken. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
200kDa
3D Structure
ModBase 3D Structure for P12036
Immunogen
Recombinant human neurofilament protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
22q12.2
Positive Control
Brain, Neuroblastoma
Applications Tested/Suitable for anti-NF-H antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-NF-H antibody
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-NF-H antibody

Formalin-fixed, paraffin-embedded human Brain stained with Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).
Mouse Anti-NF-H Monoclonal Antibody – Cat# MBS4380134

SDS-Page of anti-NF-H antibody

SDS-PAGE Analysis of Purified Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).
Mouse Anti-NF-H Monoclonal Antibody, SDS-Page – Cat# MBS4380134

Product References and Citations for anti-NF-H antibody

Shaw G et. al. European Journal of Cell Biology. 34: 130-136 (1984). Shaw G et. al. European Journal of Cell Biology. 42: 1-9 (1986).

Other Notes

Small volumes of anti-NF-H antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NF-H. It may not necessarily be applicable to this product.

NCBI GI #
32483416
NCBI GeneID
4744
NCBI Accession #
NP_066554.2
UniProt Primary Accession #
P12036
UniProt Secondary Accession #
Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
UniProt Related Accession #
P12036
GenBank Nucleotide #
NM_021076.3
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament heavy
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH; CMT2CC
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Synonym Protein Names
200 kDa neurofilament protein; Neurofilament triplet H protein
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
NCBI Protein Information
neurofilament heavy polypeptide

NCBI Summary for NF-H

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for NF-H

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Research Articles on NF-H

1. Data suggest that high CSF NfH levels are an early predictor of later brain and spinal cord atrophy in multiple sclerosis patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NF-H antibody

 Reference Product  PubMed Publications
 NEFL antibody  >3 publications with NF-H and NEFL
 PRPH antibody  >1 publications with NF-H and PRPH

Pathways associated with anti-NF-H antibody

 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with anti-NF-H antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >13 publications with NF-H and Nervous System Diseases
 Neoplasms Antibodies  >7 publications with NF-H and Neoplasms
 Brain Diseases Antibodies  >4 publications with NF-H and Brain Diseases
 Drug Toxicity Antibodies  >3 publications with NF-H and Drug Toxicity
 Death Antibodies  >3 publications with NF-H and Death
 Brain Injuries Antibodies  >2 publications with NF-H and Brain Injuries
 Disease Models, Animal Antibodies  >1 publications with NF-H and Disease Models, Animal
 Atrophy Antibodies  >1 publications with NF-H and Atrophy
 Poisoning Antibodies  >1 publications with NF-H and Poisoning
 Neurotoxicity Syndromes Antibodies  >1 publications with NF-H and Neurotoxicity Syndromes

Organs/Tissues associated with anti-NF-H antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with NF-H and Brain
 Nerve Antibodies  >7 publications with NF-H and Nerve
 Spinal Cord Antibodies  >2 publications with NF-H and Spinal Cord
 Testis Antibodies  >2 publications with NF-H and Testis
 Eye Antibodies  >2 publications with NF-H and Eye
 Ear Antibodies  >1 publications with NF-H and Ear
 Heart Antibodies  >1 publications with NF-H and Heart
 Bone Marrow Antibodies  >1 publications with NF-H and Bone Marrow
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