Mouse Anti-DOG-1 Monoclonal Antibody – Cat# MBS4380182

Formalin-fixed, paraffin-embedded human GIST stained with DOG-1 Monoclonal Antibody (DG1/1485).

DOG-1 Antibody – Mouse Monoclonal

Name: DOG-1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS4380182
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380182

MBS4380182 is an antibody targeting DOG-1. Also known as DOG-1/TMEM16A (Gastrointestinal Stromal Tumor Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DOG-1/TMEM16A, Monoclonal Antibody

Full Product Name

DOG-1/TMEM16A (Gastrointestinal Stromal Tumor Marker)

Product Gene Name

anti-DOG-1 antibody

Product Synonym Gene Name

TMEM16A

Product Synonym Names

Anoctamin 1; Calcium Activated Chloride Channel; Discovered On Gastrointestinal Stromal Tumors Protein 1; TAOS2; ORAOV2; TMEM16A

Clonality

Monoclonal

Isotype

IgG2b, kappa

Clone Number

DG1/1485

Host

Mouse

Species Reactivity

Human. Others not known.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

~114kDa

3D Structure

ModBase 3D Structure for Q5XXA6

Immunogen

Recombinant human DOG-1 protein fragment (aa 2-101)

Cellular Localization

Cell Surface and Cytoplasm

Hu-chromosome Location

11q13.3

Positive Control

Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.

Applications Tested/Suitable for anti-DOG-1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-DOG-1 antibody

FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Product References and Citations for anti-DOG-1 antibody

Espinosa I, et. al. Am J Surg Pathol 2008; 32:210-218.

Other Notes

Small volumes of anti-DOG-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DOG-1. It may not necessarily be applicable to this product.

NCBI GI #

194306539

NCBI GeneID

55107

NCBI Accession #

NP_060513.5

UniProt Primary Accession #

Q5XXA6

UniProt Secondary Accession #

Q8IYY8; Q8N7V3; A8KAM3

UniProt Related Accession #

Q5XXA6

GenBank Nucleotide #

NM_018043.5

NCBI Official Full Name

anoctamin-1

NCBI Official Synonym Full Names

anoctamin 1

NCBI Official Symbol

ANO1

NCBI Official Synonym Symbols

DOG1; TAOS2; ORAOV2; TMEM16A

UniProt Protein Name

Anoctamin-1

UniProt Synonym Protein Names

Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2

UniProt Gene Name

ANO1

UniProt Synonym Gene Names

DOG1; ORAOV2; TAOS2; TMEM16A

NCBI Protein Information

anoctamin-1

UniProt Comments for DOG-1

Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.

Research Articles on DOG-1

1. TMEM16A expression was found to correlate with greater tumor size, less Bim expression, and less apoptotic activity overall in head and neck squamous cell carcinomas (HNSCCs).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOG-1 antibody

Reference Product	PubMed Publications
KIT antibody	>63 publications with DOG-1 and KIT
PDGFRA antibody	>30 publications with DOG-1 and PDGFRA
CLCA1 antibody	>3 publications with DOG-1 and CLCA1
ORAOV1 antibody	>1 publications with DOG-1 and ORAOV1
FGF3 antibody	>1 publications with DOG-1 and FGF3

Pathways associated with anti-DOG-1 antibody

Products by Pathway	Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Orphan Transporters Pathway antibodies	Orphan Transporters Pathway Diagram
Stimuli-sensing Channels Pathway antibodies	Stimuli-sensing Channels Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-DOG-1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>8 publications with DOG-1 and Adenocarcinoma
Inflammation Antibodies	>8 publications with DOG-1 and Inflammation
Cardiovascular Diseases Antibodies	>7 publications with DOG-1 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>6 publications with DOG-1 and Breast Neoplasms
Uterine Neoplasms Antibodies	>5 publications with DOG-1 and Uterine Neoplasms
Kidney Diseases Antibodies	>4 publications with DOG-1 and Kidney Diseases
Disease Models, Animal Antibodies	>3 publications with DOG-1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>3 publications with DOG-1 and Neoplasms, Experimental
Necrosis Antibodies	>3 publications with DOG-1 and Necrosis
Prostatic Neoplasms Antibodies	>2 publications with DOG-1 and Prostatic Neoplasms

Organs/Tissues associated with anti-DOG-1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>65 publications with DOG-1 and Muscle
Intestine Antibodies	>25 publications with DOG-1 and Intestine
Stomach Antibodies	>19 publications with DOG-1 and Stomach
Vascular Antibodies	>17 publications with DOG-1 and Vascular
Lung Antibodies	>17 publications with DOG-1 and Lung
Brain Antibodies	>13 publications with DOG-1 and Brain
Kidney Antibodies	>11 publications with DOG-1 and Kidney
Salivary Gland Antibodies	>10 publications with DOG-1 and Salivary Gland
Eye Antibodies	>10 publications with DOG-1 and Eye
Pancreas Antibodies	>8 publications with DOG-1 and Pancreas