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SOX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380226

MBS4380226 is an antibody targeting SOX2. Also known as SOX2 (Transcription Factor). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SOX2, Monoclonal Antibody
Full Product Name
SOX2 (Transcription Factor)
Product Gene Name
Product Synonym Names
ANOP3; Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); SOX-2; SRY (sex determining region Y) box 2; SRY related HMG box 2; Transcription factor SOX-2; ysb
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SOX2/1791
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
34kDa
3D Structure
ModBase 3D Structure for P48431
Immunogen
Recombinant human SOX2 protein fragment (aa176-305)
Cellular Localization
Nuclear
Hu-chromosome Location
3q26.33
Positive Control
MCF-7 cells or Cervical carcinoma
Applications Tested/Suitable for anti-SOX2 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-SOX2 antibody
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-SOX2 antibody

Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1791).
Mouse Anti-SOX2 Monoclonal Antibody – Cat# MBS4380226

Immunohistochemistry (IHC) of anti-SOX2 antibody

Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1247).
Mouse Anti-SOX2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380226

Product References and Citations for anti-SOX2 antibody

Laudet, V., Stehelin, D. and Clevers, H. 1993. Ancestry and diversity of the HMG box superfamily. Nucleic Acids Res. 21: 2493-2501

Other Notes

Small volumes of anti-SOX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY-box 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
NCBI Protein Information
transcription factor SOX-2

NCBI Summary for SOX2

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
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UniProt Comments for SOX2

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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