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TNF-alpha Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380259

MBS4380259 is an antibody targeting TNF-alpha. Also known as TNF-alpha (Tumor Necrosis Factor alpha). Reactive in Human, Rat. Others not known.. Host: Rabbit. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TNF-alpha (Tumor Necrosis Factor alpha), Monoclonal Antibody
Full Product Name
TNF-alpha (Tumor Necrosis Factor alpha)
Product Gene Name
anti-TNF-alpha antibody
Product Synonym Gene Name
TNF
Product Synonym Names
APC1; Cachectin; Differentiation inducing factor (DIF); Macrophage cytotoxic factor (MCF); Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor
Clonality
Monoclonal
Isotype
IgG
Clone Number
TNF/1500R
Host
Rabbit
Species Reactivity
Human, Rat. Others not known.
Form/Format
200ug/ml of Ab purified. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Specificity
This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Non-hazardous. No MSDS required.
Molecular Weight
17kDa
3D Structure
ModBase 3D Structure for P01375
Immunogen
Recombinant full-length human TNF-alpha protein
Cellular Localization
Cytoplasmic and extracellular (secreted)
Hu-chromosome Location
6p21.33
Positive Control
HeLa, HL-60, or A431 cells. Pancreas or Histiocytoma
Applications Tested/Suitable for anti-TNF-alpha antibody
Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes for anti-TNF-alpha antibody
Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-TNF-alpha antibody

Formalin-fixed, paraffin-embedded Human Histiocytoma stained with TNF alpha Rabbit Recombinant MAb (TNF/1500R).
Rabbit Anti-TNF-alpha Monoclonal Antibody – Cat# MBS4380259

Immunohistochemistry (IHC) of anti-TNF-alpha antibody

Formalin-fixed, paraffin-embedded Human Pancreas stained with TNF alpha Rabbit Recombinant MAb (TNF/1500R).
Rabbit Anti-TNF-alpha Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380259

SDS-Page of anti-TNF-alpha antibody

SDS-PAGE Analysis Purified TNF alpha Rabbit Recombinant MAb (TNF/1500R).
Rabbit Anti-TNF-alpha Monoclonal Antibody, SDS-Page – Cat# MBS4380259

Product References and Citations for anti-TNF-alpha antibody

ebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35.

Other Notes

Small volumes of anti-TNF-alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF-alpha. It may not necessarily be applicable to this product.

NCBI GI #
25952111
NCBI GeneID
7124
NCBI Accession #
NP_000585.2
UniProt Primary Accession #
P01375
UniProt Secondary Accession #
O43647; Q9P1Q2; Q9UIV3
UniProt Related Accession #
P01375
GenBank Nucleotide #
NM_000594.3
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
NCBI Protein Information
tumor necrosis factor

NCBI Summary for TNF-alpha

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
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UniProt Comments for TNF-alpha

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).
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Research Articles on TNF-alpha

1. High TNFA expression may contribute for faster dopaminergic neuron degeneration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNF-alpha antibody

 Reference Product  PubMed Publications
 TRAF2 antibody  >917 publications with TNF-alpha and TRAF2
 TNFRSF1A antibody  >351 publications with TNF-alpha and TNFRSF1A
 IFNG antibody  >113 publications with TNF-alpha and IFNG
 ADAM17 antibody  >112 publications with TNF-alpha and ADAM17
 TNFRSF1B antibody  >90 publications with TNF-alpha and TNFRSF1B
 PRTN3 antibody  >45 publications with TNF-alpha and PRTN3
 CSF1 antibody  >15 publications with TNF-alpha and CSF1
 DCN antibody  >14 publications with TNF-alpha and DCN
 ADAM9 antibody  >10 publications with TNF-alpha and ADAM9
 BGN antibody  >6 publications with TNF-alpha and BGN

Pathways associated with anti-TNF-alpha antibody

 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram

Diseases associated with anti-TNF-alpha antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >80591 publications with TNF-alpha and Necrosis
 Inflammation Antibodies  >26751 publications with TNF-alpha and Inflammation
 Vascular Diseases Antibodies  >6430 publications with TNF-alpha and Vascular Diseases
 Lung Diseases Antibodies  >5841 publications with TNF-alpha and Lung Diseases
 Skin Diseases Antibodies  >5017 publications with TNF-alpha and Skin Diseases
 Liver Diseases Antibodies  >4388 publications with TNF-alpha and Liver Diseases
 Fibrosis Antibodies  >2046 publications with TNF-alpha and Fibrosis
 Insulin Resistance Antibodies  >2037 publications with TNF-alpha and Insulin Resistance
 Atherosclerosis Antibodies  >1997 publications with TNF-alpha and Atherosclerosis
 Pain Antibodies  >1814 publications with TNF-alpha and Pain

Organs/Tissues associated with anti-TNF-alpha antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >45739 publications with TNF-alpha and Blood
 Lung Antibodies  >8433 publications with TNF-alpha and Lung
 Brain Antibodies  >5912 publications with TNF-alpha and Brain
 Heart Antibodies  >4369 publications with TNF-alpha and Heart
 Intestine Antibodies  >3060 publications with TNF-alpha and Intestine
 Connective Tissue Antibodies  >1928 publications with TNF-alpha and Connective Tissue
 Lymph Node Antibodies  >1763 publications with TNF-alpha and Lymph Node
 Placenta Antibodies  >742 publications with TNF-alpha and Placenta
 Prostate Antibodies  >714 publications with TNF-alpha and Prostate
 Ovary Antibodies  >487 publications with TNF-alpha and Ovary
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