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Tyrosinase-Related Protein-1 (TYRP-1) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380274

MBS4380274 is an antibody targeting TYRP-1. Also known as Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker). Reactive in Human, Mouse. Others not known.. Host: Rabbit. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tyrosinase-Related Protein-1 (TYRP-1), Monoclonal Antibody
Full Product Name
Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker)
Product Gene Name
Product Synonym Gene Name
TYRP1
Product Synonym Names
5; 6 dihydroxyindole 2 carboxylic acid oxidase; 6-dihydroxyindole-2-carboxylic acid oxidase; Associated with iris pigmentation; CAS2; Catalase B (CATB); DHICA oxidase; Glycoprotein75 (GP75); Melanoma antigen gp75; Tyrosinase-related protein 1 (TYRP1); TYRRP
Clonality
Monoclonal
Isotype
IgG
Clone Number
TYRP1/1564R
Host
Rabbit
Species Reactivity
Human, Mouse. Others not known.
Form/Format
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
75kDa
3D Structure
ModBase 3D Structure for P17643
Immunogen
Recombinant full-length human TYRP1 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
9p23
Positive Control
SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
Applications Tested/Suitable for anti-TYRP-1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TYRP-1 antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
IHC-F: 5-10ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-TYRP-1 antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with TYRP1 Rabbit Recombinant MAb (TYRP1/1564R) using AEC Chromogen.
Rabbit Anti-TYRP-1 Monoclonal Antibody – Cat# MBS4380274

SDS-Page of anti-TYRP-1 antibody

SDS-PAGE Analysis of Purified TYRP1 Rabbit Recombinant Monoclonal Antibody (TYRP1/1564R) using AEC Chromogen.
Rabbit Anti-TYRP-1 Monoclonal Antibody, SDS-Page – Cat# MBS4380274

Product References and Citations for anti-TYRP-1 antibody

Orlow, S.J., et al. 1994. High-molecular-weight forms of tyrosinase and the tyrosinase-related proteins: evidence for a melanogenic complex. J. Invest. Dermatol. 103: 196-201.

Other Notes

Small volumes of anti-TYRP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TYRP-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
NCBI Official Synonym Full Names
tyrosinase related protein 1
NCBI Official Symbol
TYRP1
NCBI Official Synonym Symbols
TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
UniProt Protein Name
5,6-dihydroxyindole-2-carboxylic acid oxidase
UniProt Synonym Protein Names
Catalase B; Glycoprotein 75
UniProt Gene Name
TYRP1
UniProt Synonym Gene Names
; TRP-1; TRP1
NCBI Protein Information
5,6-dihydroxyindole-2-carboxylic acid oxidase

NCBI Summary for TYRP-1

This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]

UniProt Comments for TYRP-1

Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu2+ ions (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:22556244, PubMed:16704458). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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