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CD86 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380337

MBS4380337 is an antibody targeting CD86. Also known as CD86 (Dendritic Cells Maturation Marker). Reactive in Human, Mouse, Rat.. Host: Rabbit. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD86, Monoclonal Antibody
Full Product Name
CD86 (Dendritic Cells Maturation Marker)
Product Gene Name
Product Synonym Names
Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2
Clonality
Monoclonal
Isotype
IgG
Clone Number
C86/2160R
Host
Rabbit
Species Reactivity
Human, Mouse, Rat.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Preparation and Storage
Store at 2 to 8 degree C. Non-hazardous. No MSDS required.
Molecular Weight
70kDa
3D Structure
ModBase 3D Structure for P4208
Immunogen
Recombinant full-length human CD86 protein
Cellular Localization
Cell Surface
Hu-chromosome Location
3q13.33
Positive Control
293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
Applications Tested/Suitable for anti-CD86 antibody
Flow Cytometry (FC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-CD86 antibody
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined

Immunohistochemistry (IHC) of anti-CD86 antibody

Formalin-fixed, paraffin-embedded Human Colon stained with CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).
Rabbit Anti-CD86 Monoclonal Antibody – Cat# MBS4380337

Western Blot (WB) of anti-CD86 antibody

Western Blot Analysis of Ramos cell lysate using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).
Rabbit Anti-CD86 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380337

Flow Cytometry (FC) of anti-CD86 antibody

Flow Cytometric Analysis of Ramos cells using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Rabbit Anti-CD86 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS4380337

Immunofluorescence (IF) of anti-CD86 antibody

Immunofluorescent Staining of Raji cells using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R) followed by goat anti-rabbit IgG conjugated with CF488 (green). Nuclei are stained with Red Dot.
Rabbit Anti-CD86 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS4380337

SDS-Page of anti-CD86 antibody

SDS-PAGE Analysis of Purified CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).
Rabbit Anti-CD86 Monoclonal Antibody, SDS-Page – Cat# MBS4380337

Product References and Citations for anti-CD86 antibody

Engel P et. al. Blood. 1994; 84(5):1402-7.

Other Notes

Small volumes of anti-CD86 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD86. It may not necessarily be applicable to this product.

NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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