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GAD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380441

MBS4380441 is an antibody targeting GAD1. Also known as GAD1/GAD67 (GABAergic Neuronal Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GAD1/GAD67, Monoclonal Antibody
Full Product Name
GAD1/GAD67 (GABAergic Neuronal Marker)
Product Gene Name
Product Synonym Gene Name
GAD67
Product Synonym Names
67kDa glutamic acid decarboxylase; CPSQ1; DCE1; GAD67; GAD1; Glutamate decarboxylase 1; SCP
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
GAD1/2391
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
Molecular Weight
67kDa
3D Structure
ModBase 3D Structure for Q99259
Immunogen
Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
2q31.1
Positive Control
K-562 or HEK293 Cells. Pancreas
Applications Tested/Suitable for anti-GAD1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-GAD1 antibody
ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-GAD1 antibody

Formalin-fixed, paraffin-embedded human Brain stained with GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).
Mouse Anti-GAD1 Monoclonal Antibody – Cat# MBS4380441

SDS-Page of anti-GAD1 antibody

SDS-PAGE Analysis Purified GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).Confirmation of Integrity and Purity of Antibody.
Mouse Anti-GAD1 Monoclonal Antibody, SDS-Page – Cat# MBS4380441

Western Blot (WB) of anti-GAD1 antibody

Western Blot Analysis of human Brain tissue lysate using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).
Mouse Anti-GAD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380441

Testing Data of anti-GAD1 antibody

Analysis of Protein Array containing more than 19, 000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-GAD1 Monoclonal Antibody, Testing Data – Cat# MBS4380441

Product References and Citations for anti-GAD1 antibody

Kaufman DL, Erlander MG, Clare-Salzler M, Atkinson MA, Maclaren NK, Tobin AJ. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus. J Clin Invest. 1992 Jan; 89(1):283-92.

Other Notes

Small volumes of anti-GAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutamate decarboxylase 1 isoform GAD67
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
UniProt Gene Name
GAD1
UniProt Synonym Gene Names
GAD; GAD67; GAD-67
NCBI Protein Information
glutamate decarboxylase 1

NCBI Summary for GAD1

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
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UniProt Comments for GAD1

Catalyzes the production of GABA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAD1 antibody

 Reference Product  PubMed Publications
 GAD2 antibody  >40 publications with GAD1 and GAD2
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