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Granzyme B (GZMB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380448

MBS4380448 is an antibody targeting GZMB. Also known as Granzyme B (NK/T-Cell Lymphoma Marker). Reactive in Human. Others not known. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Granzyme B (GZMB), Monoclonal Antibody
Full Product Name
Granzyme B (NK/T-Cell Lymphoma Marker)
Product Gene Name
Product Synonym Names
Cathepsin G-like 1; CCPI; CGL1; CSPB; CTLA-1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; Granzyme B; Granzyme-2; GRB; Gzmb; Human lymphocyte protein (Hlp); Lymphocyte protease; SECT; T-cell serine protease 1-3E
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
GZMB/2403
Host
Mouse
Species Reactivity
Human. Others not known
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkins Disease.
Molecular Weight
29-32kDa
3D Structure
ModBase 3D Structure for P10144
Immunogen
Recombinant fragment of human GZMB protein (around aa 73-187)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
14q11.2
Positive Control
A431 cells. Tonsil, Spleen or Hodgkins Lymphoma
Applications Tested/Suitable for anti-GZMB antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-GZMB antibody
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-GZMB antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Granzyme B Mouse Monoclonal Antibody (GZMB/2403).
Mouse Anti-GZMB Monoclonal Antibody – Cat# MBS4380448

Immunohistochemistry (IHC) of anti-GZMB antibody

Formalin-fixed, paraffin-embedded human Spleen stained with Granzyme B Mouse Monoclonal Antibody (GZMB/2403).
Mouse Anti-GZMB Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380448

SDS-Page of anti-GZMB antibody

SDS-PAGE Analysis Purified Granzyme B Mouse Monoclonal Antibody (GZMB/2403).Confirmation of Integrity and Purity of Antibody.
Mouse Anti-GZMB Monoclonal Antibody, SDS-Page – Cat# MBS4380448

Testing Data of anti-GZMB antibody

Analysis of Protein Array containing >19, 000 full-length human proteins using Granzyme B Mouse Monoclonal Antibody (GZMB/2403) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-GZMB Monoclonal Antibody, Testing Data – Cat# MBS4380448

Product References and Citations for anti-GZMB antibody

Shresta, S., et al. 1995. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells. Proc. Natl. Acad. Sci. USA 92: 5679-5683.

Other Notes

Small volumes of anti-GZMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
granzyme B isoform 1 preproprotein
NCBI Official Synonym Full Names
granzyme B
NCBI Official Symbol
GZMB
NCBI Official Synonym Symbols
C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
UniProt Protein Name
Granzyme B
UniProt Synonym Protein Names
C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
Protein Family
UniProt Gene Name
GZMB
UniProt Synonym Gene Names
CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
NCBI Protein Information
granzyme B

NCBI Summary for GZMB

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
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UniProt Comments for GZMB

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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