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Cytokeratin, Multi (KRT4) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438045

MBS438045 is an antibody targeting KRT4. Also known as Cytokeratin, Multi (Epithelial Marker) Mouse Monoclonal Antibody. Reactive in Human, Cow, Rat, Mouse, Guinea Pig, Frog, Goat, Marmoset, Pig.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin, Multi (KRT4), Monoclonal Antibody
Full Product Name
Cytokeratin, Multi (Epithelial Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-KRT4 antibody
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
C11
Host
Mouse
Species Reactivity
Human, Cow, Rat, Mouse, Guinea Pig, Frog, Goat, Marmoset, Pig.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Non-hazardous. No MSDS required.
Molecular Weight
Multiple
OMIM
123940
3D Structure
ModBase 3D Structure for P19013
Cellular Localization
Cytoplasmic
Immunogen
Keratin-enriched preparation from cultured human A431
Positive Control
A431 cells, Skin, Colon carcinoma
Applications Tested/Suitable for anti-KRT4 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-KRT4 antibody
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined

Immunohistochemistry (IHC) of anti-KRT4 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (C11).
Mouse Anti-KRT4 Monoclonal Antibody – Cat# MBS438045

Immunohistochemistry (IHC) of anti-KRT4 antibody

Mouse Anti-KRT4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438045

Immunofluorescence (IF) of anti-KRT4 antibody

Mouse Anti-KRT4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS438045

Flow Cytometry (FC) of anti-KRT4 antibody

Mouse Anti-KRT4 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438045

Product References and Citations for anti-KRT4 antibody

Kovaik et al. Int.J.Cancer, Suppl. 3: 50-55, (1988).,Folia Biol., 35(6), 373-382, (1989),Vojtšek et al. Neoplasma 37(3), 333-342 (1990).,Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).,Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79

Other Notes

Small volumes of anti-KRT4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT4. It may not necessarily be applicable to this product.

NCBI GI #
82654947
NCBI GeneID
3851
NCBI Accession #
P19013.4
UniProt Primary Accession #
P19013
UniProt Secondary Accession #
Q6GTR8; Q96LA7; Q9BTL1; F8VS64
NCBI Official Full Name
Keratin, type II cytoskeletal 4
UniProt Protein Name
Keratin, type II cytoskeletal 4
UniProt Synonym Protein Names
Cytokeratin-4; CK-4; Keratin-4; K4; Type-II keratin Kb4
Protein Family
Keratin
UniProt Gene Name
KRT4
UniProt Synonym Gene Names
CYK4; CK-4; K4
UniProt Entry Name
K2C4_HUMAN

UniProt Comments for KRT4

K4: Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13. Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa. Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; intermediate filament cytoskeleton; keratin filament; nucleus

Molecular Function: protein binding

Biological Process: cytoskeleton organization and biogenesis; epithelial cell differentiation; negative regulation of epithelial cell proliferation

Disease: White Sponge Nevus 1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-KRT4 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >13 publications with KRT4 and Skin
 Mouth Antibodies  >6 publications with KRT4 and Mouth
 Lung Antibodies  >2 publications with KRT4 and Lung
 Eye Antibodies  >2 publications with KRT4 and Eye
 Embryonic Tissue Antibodies  >2 publications with KRT4 and Embryonic Tissue
 Esophagus Antibodies  >2 publications with KRT4 and Esophagus
 Muscle Antibodies  >1 publications with KRT4 and Muscle
 Uncharacterized Tissue Antibodies  >1 publications with KRT4 and Uncharacterized Tissue
 Heart Antibodies  >1 publications with KRT4 and Heart
 Brain Antibodies  >1 publications with KRT4 and Brain
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