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Pd-L1 / PDCD1LG1 / CD274 / B7-H1 (CD274) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380524

MBS4380524 is an antibody targeting CD274. Also known as PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Mouse Monoclonal Antibody [Clone PDL1/2746]. Reactive in Human, Mouse. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PD-L1 / PDCD1LG1 / CD274 / B7-H1, Antibody
Full Product Name
PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Mouse Monoclonal Antibody [Clone PDL1/2746]
Product Gene Name
anti-CD274 antibody
Product Synonym Names
B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1
Isotype
IgG2b, kappa
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. recruitment of CTLA4 by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acid type I transmembrane protein, which is 20% and 15% identical to B7-1 and B7-2, respectively, has immunoglobulin V-like and C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2 protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. Constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L system could provide unique negative signaling to help prevent autoimmune diseases.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
37-50kDa
3D Structure
ModBase 3D Structure for Q9NZQ7
Immunogen
Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
Cellular Localization
Cell Surface and Cytoplasmic
Positive Control
HEK293 or HepG2. Heart, Placenta, Spleen or Tonsil.
Application Notes for anti-CD274 antibody
ELISA (For coating, order antibody without BSA); Western Blotting (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined

Immunohistochemistry (IHC) of anti-CD274 antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with PD-L1 Mouse Monoclonal Antibody (PDL1/2746).
Mouse Anti-CD274 Antibody – Cat# MBS4380524

Immunohistochemistry (IHC) of anti-CD274 antibody

Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with PD-L1 Mouse Monoclonal Antibody (PDL1/2746).
Mouse Anti-CD274 Antibody, Immunohistochemistry (IHC) – Cat# MBS4380524

SDS-Page of anti-CD274 antibody

SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-CD274 Antibody, SDS-Page – Cat# MBS4380524

Western Blot (WB) of anti-CD274 antibody

Western Blot Analysis of human HEK293 and HepG2 Cell Lysate using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).
Mouse Anti-CD274 Antibody, Western Blot (WB) – Cat# MBS4380524

Western Blot (WB) of anti-CD274 antibody

Western Blot Analysis of human RAW, A549, THP1, MOLT-4, Raji, and MDA-MB-231 Cell Lysate using PD-L1 Mouse Monoclonal Antibody (PDL1/2746).
Mouse Anti-CD274 Antibody, Western Blot (WB) – Cat# MBS4380524

Flow Cytometry (FC/FACS) of anti-CD274 antibody

Flow Cytometric Analysis of Human Jurkat Cells. using PD-L1 Mouse Monoclonal Antibody (PDL1/2746) followed by Goat anti-mouse IgG-CF488 (Orange); Cells alone (Blue); Isotype Control (Red).
Mouse Anti-CD274 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS4380524

Immunofluorescence (IF) of anti-CD274 antibody

Immunofluorescence Analysis of Human Jurkat Cells labeling PD-L1 with PD-L1 Mouse Monoclonal Antibody (PDL1/2746) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Mouse Anti-CD274 Antibody, Immunofluorescence (IF) – Cat# MBS4380524

Testing Data of anti-CD274 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-CD274 Antibody, Testing Data – Cat# MBS4380524

Product References and Citations for anti-CD274 antibody

1. Dong, H., et al. 1999. Nat. Med. 5: 1365-1369.

Other Notes

Small volumes of anti-CD274 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.

NCBI GeneID
29126
UniProt Primary Accession #
Q9NZQ7
Protein Family
Programmed cell death

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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