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Topoisomerase I, Mitochondrial (TOP1MT) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380530

MBS4380530 is an antibody targeting TOP1MT. Also known as Topoisomerase I, Mitochondrial (TOP1MT). Reactive in Human. Others not known.. Host: Rabbit. Recombinant Rabbit Monoclonal Antibody More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Topoisomerase I, Mitochondrial (TOP1MT), Monoclonal Antibody
Full Product Name
Topoisomerase I, Mitochondrial (TOP1MT)
Product Gene Name
Product Synonym Names
DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase
Clonality
Monoclonal
Isotype
IgG
Clone Number
TOP1MT/2883R
Host
Rabbit
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
70kDa
3D Structure
ModBase 3D Structure for Q969P6
Immunogen
Recombinant full-length human TOP1MT protein
Positive Control
A431 Cells. Heart, Skeletal Muscle, Brain or Fetal Liver.
Cellular Localization
Cytoplasmic (Mitochondria)
Chromosome Location
8q24.3
Applications Tested/Suitable for anti-TOP1MT antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-TOP1MT antibody
Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-TOP1MT antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Topo I Rabbit Recombinant Monoclonal Antibody (TOP1MT/2883R).
Rabbit Anti-TOP1MT Monoclonal Antibody – Cat# MBS4380530

SDS-Page of anti-TOP1MT antibody

SDS-PAGE Analysis Purified Topo I Rabbit Recombinant Monoclonal Antibody (TOP1MT/2883R). Confirmation of Purity and Integrity of Antibody.
Rabbit Anti-TOP1MT Monoclonal Antibody, SDS-Page – Cat# MBS4380530

Product References and Citations for anti-TOP1MT antibody

Zhang, H., et al. Biochimie 89: 474-481.; Zhang, H., et al. Proc. Natl. Acad. Sci. USA 98: 10608-10613.

Other Notes

Small volumes of anti-TOP1MT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TOP1MT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA topoisomerase I, mitochondrial isoform 2
NCBI Official Synonym Full Names
DNA topoisomerase I mitochondrial
NCBI Official Symbol
TOP1MT
UniProt Protein Name
DNA topoisomerase I, mitochondrial
Protein Family
UniProt Gene Name
TOP1MT
UniProt Synonym Gene Names
TOP1mt
NCBI Protein Information
DNA topoisomerase I, mitochondrial

NCBI Summary for TOP1MT

This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
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UniProt Comments for TOP1MT

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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