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Carboxypeptidase A1/CPA1 (CPA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380533

MBS4380533 is an antibody targeting CPA1. Also known as Carboxypeptidase A1/CPA1 (Pancreatic Cancer Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carboxypeptidase A1/CPA1, Monoclonal Antibody
Full Product Name
Carboxypeptidase A1/CPA1 (Pancreatic Cancer Marker)
Product Gene Name
Product Synonym Names
Carboxypeptidase A1 (pancreatic); CPA1; Pancreatic Carboxypeptidase A1; Procarboxypeptidase A1 pancreatic
Clonality
Monoclonal
Isotype
IgG, kappa
Clone Number
CPA1/2711
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Human pancreatic procarboxypeptidase A exists as three different active forms, two of which are designated carboxypeptidase A1 (CPA1) and carboxypeptidase A2 (CPA2). CPA1, also known as CPA, is a 419 amino acid secreted monomeric protein that is highly expressed in pancreatic tissue. Functioning as a pancreatic exopeptidase, CPA1 uses zinc as a cofactor to catalyze the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Via its catalytic activity, CPA1 is also thought to be involved in zymogen (proenzyme) inhibition, probably functioning to block enzyme activation pathways. Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
47kDa
3D Structure
ModBase 3D Structure for P15085
Immunogen
Recombinant full-length human CPA1 protein
Positive Control
Pancreas
Cellular Localization
Secreted
Chromosome Location
7q32.3
Applications Tested/Suitable for anti-CPA1 antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-CPA1 antibody
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-CPA1 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Mouse Monoclonal Antibody (CPA1/2711).
Mouse Anti-CPA1 Monoclonal Antibody – Cat# MBS4380533

Immunohistochemistry (IHC) of anti-CPA1 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Mouse Monoclonal Antibody (CPA1/2711).
Mouse Anti-CPA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380533

Testing Data of anti-CPA1 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Carboxypeptidase A1/CPA1 Mouse Monoclonal Antibody (CPA1/2711). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-CPA1 Monoclonal Antibody, Testing Data – Cat# MBS4380533

Product References and Citations for anti-CPA1 antibody

Stewart, E.A., et al. 1990. Human carboxypeptidase A identifies a BglII RFLP and maps to 7q31-qter. Am. J. Hum. Genet. 46: 795-800.

Other Notes

Small volumes of anti-CPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CPA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carboxypeptidase A1 preproprotein
NCBI Official Synonym Full Names
carboxypeptidase A1
NCBI Official Symbol
CPA1
NCBI Official Synonym Symbols
CPA
UniProt Protein Name
Carboxypeptidase A1
Protein Family
UniProt Gene Name
CPA1
UniProt Synonym Gene Names
CPA
NCBI Protein Information
carboxypeptidase A1

NCBI Summary for CPA1

This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. [provided by RefSeq, Jan 2015]
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UniProt Comments for CPA1

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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