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Aldo-Keto Reductase Family 1 Member B1 (AKR1B1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380556

MBS4380556 is an antibody targeting AKR1B1. Also known as Aldo-keto Reductase Family 1 Member B1 (Adrenal Marker). Reactive in Human. Others not tested.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aldo-keto Reductase Family 1 Member B1 (AKR1B1), Monoclonal Antibody
Full Product Name
Aldo-keto Reductase Family 1 Member B1 (Adrenal Marker)
Product Gene Name
Product Synonym Names
AKR1B1; Aldehyde reductase; aldehyde reductase 1; Aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); Aldose reductase; AR; Lii5-2 CTCL tumor antigen; low Km aldose reductase
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CPTC-AKR1B1-2
Host
Mouse
Species Reactivity
Human. Others not tested.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
AKR1B1, also deisgnated as aldose reductase, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. It has also been shown to have decreased expression in adrenocortical cancer, and possibly play a role in adrenal tumorigenesis. It has been suggested that AKR1B1 could be investigated as a marker of malignancy for adrenal tumor.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
37kDa
3D Structure
ModBase 3D Structure for P15121
Immunogen
Recombinant human full-length AKR1B1protein
Positive Control
293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human colon carcinoma tissue.
Cellular Localization
Cytoplasmic
Chromosome Location
7q33
Applications Tested/Suitable for anti-AKR1B1 antibody
Western Blot (WB), Immunohistology (Formalin-Fixed)
Application Notes for anti-AKR1B1 antibody
WB: 0.5-1.0ug/ml
Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

SDS-Page of anti-AKR1B1 antibody

SDS-PAGE Analysis Purified AKR1B1 Mouse Monoclonal Antibody (CPTC-AKR1B1-2). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-AKR1B1 Monoclonal Antibody – Cat# MBS4380556

Testing Data of anti-AKR1B1 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Aldo-keto Reductase Family 1 Member B1 Mouse Monoclonal Antibody (CPTC-AKR1B1-2).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-AKR1B1 Monoclonal Antibody, Testing Data – Cat# MBS4380556

Product References and Citations for anti-AKR1B1 antibody

Bohren, K.M., et al. J. Biol. Chem. 264: 9547-9551.; Lefrançois-Martinez, Anne-Marie, et al. OUP Academic, Oxford University Press.

Other Notes

Small volumes of anti-AKR1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AKR1B1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
aldose reductase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member B
NCBI Official Symbol
AKR1B1
NCBI Official Synonym Symbols
AR; ADR; ALR2; ALDR1
UniProt Protein Name
Aldose reductase
UniProt Synonym Protein Names
Aldehyde reductase; Aldo-keto reductase family 1 member B1
Protein Family
UniProt Gene Name
AKR1B1
UniProt Synonym Gene Names
ALDR1; AR
NCBI Protein Information
aldose reductase

NCBI Summary for AKR1B1

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
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UniProt Comments for AKR1B1

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AKR1B1 antibody

 Reference Product  PubMed Publications
 SORD antibody  >3 publications with AKR1B1 and SORD
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