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AMACR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380561

MBS4380561 is an antibody targeting AMACR. Also known as AMACR/p504S (Prostate Cancer Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AMACR/p504S, Monoclonal Antibody
Full Product Name
AMACR/p504S (Prostate Cancer Marker)
Product Gene Name
anti-AMACR antibody
Product Synonym Names
Alpha-methylacyl-CoA Racemase; CBAS4; Da1-8; Macr1; RACE; RM
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
AMACR/1864
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
42kDa
3D Structure
ModBase 3D Structure for Q9UHK6
Immunogen
Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)
Positive Control
HEK Cells or Prostate Adenocarcinoma
Cellular Localization
Cytoplasmic (granular)
Chromosome Location
5p13.3
Applications Tested/Suitable for anti-AMACR antibody
Western Blot (WB), Immunohistology (Formalin-Fixed)
Application Notes for anti-AMACR antibody
WB: 0.5-1.0ug/ml
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-AMACR antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).
Mouse Anti-AMACR Monoclonal Antibody – Cat# MBS4380561

Immunohistochemistry (IHC) of anti-AMACR antibody

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).
Mouse Anti-AMACR Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380561

Western Blot (WB) of anti-AMACR antibody

Western Blot (1) Recombinant AMACR and (2) Human Kidney lysate using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).
Mouse Anti-AMACR Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380561

Western Blot (WB) of anti-AMACR antibody

Western Blot of Human Prostate Cancer PC3 Cell lysate AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).
Mouse Anti-AMACR Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380561

SDS-Page of anti-AMACR antibody

SDS-PAGE Analysis Purified AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864) Confirmation of Purity and Integrity of Antibody.
Mouse Anti-AMACR Monoclonal Antibody, SDS-Page – Cat# MBS4380561

Testing Data of anti-AMACR antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-AMACR Monoclonal Antibody, Testing Data – Cat# MBS4380561

Product References and Citations for anti-AMACR antibody

Jiang Z et. al. Human Pathology. 2003; 34(8):792

Other Notes

Small volumes of anti-AMACR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMACR. It may not necessarily be applicable to this product.

NCBI GI #
266456254
NCBI GeneID
23600
NCBI Accession #
NP_001161067.1
UniProt Primary Accession #
Q9UHK6
UniProt Secondary Accession #
O43673; Q3KT79; Q96GH1; Q9Y3Q1; A5YM47; B8Y916; B8Y918; F8W9N1
UniProt Related Accession #
Q9UHK6
GenBank Nucleotide #
NM_001167595.1
NCBI Official Full Name
alpha-methylacyl-CoA racemase isoform 3
NCBI Official Synonym Full Names
alpha-methylacyl-CoA racemase
NCBI Official Symbol
AMACR
NCBI Official Synonym Symbols
RM; RACE; CBAS4; P504S; AMACRD
UniProt Protein Name
Alpha-methylacyl-CoA racemase
UniProt Synonym Protein Names
2-methylacyl-CoA racemase
Protein Family
Alpha-methylacyl-CoA racemase
UniProt Gene Name
AMACR
NCBI Protein Information
alpha-methylacyl-CoA racemase

NCBI Summary for AMACR

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
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UniProt Comments for AMACR

Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.

Research Articles on AMACR

1. High expression of AMACR was not only a key adverse prognostic factor but also a potential therapeutic target in oral squamous cell carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AMACR antibody

 Reference Product  PubMed Publications
 OR51E2 antibody  >3 publications with AMACR and OR51E2
 ACOX2 antibody  >3 publications with AMACR and ACOX2
 ACOX3 antibody  >1 publications with AMACR and ACOX3
 OR51E1 antibody  >1 publications with AMACR and OR51E1

Pathways associated with anti-AMACR antibody

 Products by Pathway  Pathway Diagram
 Beta-oxidation Of Pristanoyl-CoA Pathway antibodies  Beta-oxidation Of Pristanoyl-CoA Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Peroxisomal Lipid Metabolism Pathway antibodies  Peroxisomal Lipid Metabolism Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram

Diseases associated with anti-AMACR antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >299 publications with AMACR and Carcinoma
 Prostatic Neoplasms Antibodies  >255 publications with AMACR and Prostatic Neoplasms
 Kidney Neoplasms Antibodies  >102 publications with AMACR and Kidney Neoplasms
 Kidney Diseases Antibodies  >94 publications with AMACR and Kidney Diseases
 Adenoma Antibodies  >56 publications with AMACR and Adenoma
 Hyperplasia Antibodies  >44 publications with AMACR and Hyperplasia
 Colorectal Neoplasms Antibodies  >36 publications with AMACR and Colorectal Neoplasms
 Neoplasm Metastasis Antibodies  >25 publications with AMACR and Neoplasm Metastasis
 Precancerous Conditions Antibodies  >22 publications with AMACR and Precancerous Conditions
 Necrosis Antibodies  >17 publications with AMACR and Necrosis

Organs/Tissues associated with anti-AMACR antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >304 publications with AMACR and Prostate
 Kidney Antibodies  >112 publications with AMACR and Kidney
 Blood Antibodies  >56 publications with AMACR and Blood
 Liver Antibodies  >24 publications with AMACR and Liver
 Bladder Antibodies  >23 publications with AMACR and Bladder
 Lung Antibodies  >18 publications with AMACR and Lung
 Lymph Antibodies  >17 publications with AMACR and Lymph
 Lymph Node Antibodies  >16 publications with AMACR and Lymph Node
 Intestine Antibodies  >13 publications with AMACR and Intestine
 Muscle Antibodies  >13 publications with AMACR and Muscle
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