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Cytokeratin 8/18 (KRT8) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438060

MBS438060 is an antibody targeting KRT8. Also known as Cytokeratin 8/18 Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 8/18 (KRT8), Monoclonal Antibody
Full Product Name
Cytokeratin 8/18 Mouse Monoclonal Antibody
Product Gene Name
anti-KRT8 antibody
Product Synonym Gene Name
KRT18
Product Synonym Names
CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18 Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18
Clonality
Monoclonal
Isotype
IgG
Clone Number
K8.8 + DC10
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
52.5kDa (CK8); 45kDa (CK18)
OMIM
148060
3D Structure
ModBase 3D Structure for P05787
Cellular Localization
Cytoplasmic
Immunogen
Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Hu-Chromosome Location
17q21.2 (CK8); 12q13.13 (CK18)
Positive Control
MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Applications Tested/Suitable for anti-KRT8 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-KRT8 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-KRT8 antibody

Formalin-fixed, paraffin-embedded human Ovarian Cancer stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).
Mouse Anti-KRT8 Monoclonal Antibody – Cat# MBS438060

Immunohistochemistry (IHC) of anti-KRT8 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).
Mouse Anti-KRT8 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438060

Product References and Citations for anti-KRT8 antibody

Angus B et. al. J Path, 153:377-384, 1987. Angus B et. al. J Path, 155:71-75, 198

Other Notes

Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.

NCBI GI #
372466577
NCBI GeneID
3856
NCBI Accession #
NP_001243222.1
UniProt Primary Accession #
P05787
UniProt Secondary Accession #
Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
UniProt Related Accession #
P05787
GenBank Nucleotide #
NM_001256293.1
NCBI Official Full Name
keratin, type II cytoskeletal 8 isoform 2
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Synonym Protein Names
Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Protein Family
Keratin
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8; CK-8; K8
UniProt Entry Name
K2C8_HUMAN
NCBI Protein Information
keratin, type II cytoskeletal 8

NCBI Summary for KRT8

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for KRT8

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; intermediate filament; nucleus

Molecular Function: protein binding

Disease: Cirrhosis, Familial
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Research Articles on KRT8

1. The reactivity of rheumatoid arthritis (RA) serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus patients, confirming K8 as a novel autoantigen of RA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KRT8 antibody

 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Signaling Mediated By P38-alpha And P38-beta Pathway antibodies  Signaling Mediated By P38-alpha And P38-beta Pathway Diagram

Diseases associated with anti-KRT8 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >43 publications with KRT8 and Liver Diseases
 Breast Neoplasms Antibodies  >18 publications with KRT8 and Breast Neoplasms
 Adenocarcinoma Antibodies  >17 publications with KRT8 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >14 publications with KRT8 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >14 publications with KRT8 and Disease Models, Animal
 Fibrosis Antibodies  >12 publications with KRT8 and Fibrosis
 Liver Cirrhosis Antibodies  >10 publications with KRT8 and Liver Cirrhosis
 Inflammation Antibodies  >10 publications with KRT8 and Inflammation
 Liver Neoplasms Antibodies  >10 publications with KRT8 and Liver Neoplasms
 Drug-Induced Liver Injury Antibodies  >9 publications with KRT8 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-KRT8 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >56 publications with KRT8 and Liver
 Skin Antibodies  >19 publications with KRT8 and Skin
 Embryonic Tissue Antibodies  >17 publications with KRT8 and Embryonic Tissue
 Bone Antibodies  >12 publications with KRT8 and Bone
 Mouth Antibodies  >9 publications with KRT8 and Mouth
 Kidney Antibodies  >9 publications with KRT8 and Kidney
 Lung Antibodies  >9 publications with KRT8 and Lung
 Prostate Antibodies  >7 publications with KRT8 and Prostate
 Vascular Antibodies  >6 publications with KRT8 and Vascular
 Mammary Gland Antibodies  >6 publications with KRT8 and Mammary Gland
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