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Cytokeratin 15 (KRT15) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380610

MBS4380610 is an antibody targeting KRT15. Also known as Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 15 (KRT15), Monoclonal Antibody
Full Product Name
Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
Product Gene Name
Product Synonym Names
CK15; Cytokeratin 15; K1CO; Ka15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15
Clonality
Monoclonal
Isotype
IgG
Clone Number
KRT15/2554
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
52kDa
3D Structure
ModBase 3D Structure for P19012
Immunogen
Recombinant full-length human KRT15 protein
Positive Control
HeLa or A431 Cells. Skin or Colon.
Cellular Localization
Cytoplasmic
Chromosome Location
17q21.2
Applications Tested/Suitable for anti-KRT15 antibody
Immunohistology (Frozen & Formalin-Fixed)
Application Notes for anti-KRT15 antibody
Immunohistology (Frozen & Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of Formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-KRT15 antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2554).
Mouse Anti-KRT15 Monoclonal Antibody – Cat# MBS4380610

Immunohistochemistry (IHC) of anti-KRT15 antibody

Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2554).
Mouse Anti-KRT15 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380610

Immunohistochemistry (IHC) of anti-KRT15 antibody

Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2554).
Mouse Anti-KRT15 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380610

SDS-Page of anti-KRT15 antibody

SDS-PAGE Analysis Purified Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2554). Confirmation of Purity and Integrity.
Mouse Anti-KRT15 Monoclonal Antibody, SDS-Page – Cat# MBS4380610

Testing Data of anti-KRT15 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2554). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-KRT15 Monoclonal Antibody, Testing Data – Cat# MBS4380610

Product References and Citations for anti-KRT15 antibody

Yu-Hong Shen, et al. Asian Pac J Cancer Prev, 17 (9), 4217-4222.

Other Notes

Small volumes of anti-KRT15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 15
NCBI Official Synonym Full Names
keratin 15
NCBI Official Symbol
KRT15
NCBI Official Synonym Symbols
K15; CK15; K1CO
UniProt Protein Name
Keratin, type I cytoskeletal 15
UniProt Synonym Protein Names
Cytokeratin-15; CK-15; Keratin-15; K15
Protein Family
UniProt Gene Name
KRT15
UniProt Synonym Gene Names
KRTB; CK-15; K15
NCBI Protein Information
keratin, type I cytoskeletal 15

NCBI Summary for KRT15

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT15

MiscellaneousThere are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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