• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Melanoma Antigen Family A, 4/MAGEA4 (MAGEA4) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380617

MBS4380617 is an antibody targeting MAGEA4. Also known as Melanoma Antigen Family A, 4/MAGEA4. Reactive in Human. Others not tested.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Melanoma Antigen Family A, 4/MAGEA4, Monoclonal Antibody
Full Product Name
Melanoma Antigen Family A, 4/MAGEA4
Product Gene Name
Product Synonym Names
CT1.4; MAGE-41; MAGE-X2; MAGE4; MAGE4A; MAGE4B; cancer/testis antigen 1.4; Melanoma-associated antigen 4
Clonality
Monoclonal
Isotype
IgG2c
Clone Number
CPTC-MAGEA4-1
Host
Mouse
Species Reactivity
Human. Others not tested.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
The melanoma-associated antigen (MAGE) family consists of a number of antigens recognized by cytotoxic T lymphocytes. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types, but not in normal tissues, with the exception of testis and placenta.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
45-50kDa
3D Structure
ModBase 3D Structure for P43358
Immunogen
Recombinant human full-length protein
Positive Control
HepG2 cell lysate; melanoma, testes and placenta.
Cellular Localization
Cytoplasmic and nuclear.
Chromosome Location
Xq28
Applications Tested/Suitable for anti-MAGEA4 antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-MAGEA4 antibody
Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-MAGEA4 antibody

Formalin-fixed, paraffin-embedded human Testis stained with MAGEA4 Mouse Monoclonal Antibody (CPTC-MAGEA4-1).
Mouse Anti-MAGEA4 Monoclonal Antibody – Cat# MBS4380617

Testing Data of anti-MAGEA4 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using MAGEA4 Mouse Monoclonal Antibody (CPTC-MAGEA4-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-MAGEA4 Monoclonal Antibody, Testing Data – Cat# MBS4380617

Product References and Citations for anti-MAGEA4 antibody

Rogner, UC, et al. The Melanoma Antigen Gene (MAGE) Family Is Clustered in the Chromosomal Band Xq28. Genomics., U.S. National Library of Medicine, 10 Oct. 1995.

Other Notes

Small volumes of anti-MAGEA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAGEA4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
melanoma-associated antigen 4
NCBI Official Synonym Full Names
MAGE family member A4
NCBI Official Symbol
MAGEA4
NCBI Official Synonym Symbols
CT1.4; MAGE4; MAGE4A; MAGE4B; MAGE-41; MAGE-X2
UniProt Protein Name
Melanoma-associated antigen 4
UniProt Synonym Protein Names
Cancer/testis antigen 1.4; CT1.4; MAGE-4 antigen; MAGE-41 antigen; MAGE-X2 antigen
UniProt Gene Name
MAGEA4
UniProt Synonym Gene Names
MAGE4; CT1.4
NCBI Protein Information
melanoma-associated antigen 4

NCBI Summary for MAGEA4

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for MAGEA4

Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000