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Myoglobin (MB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380618

MBS4380618 is an antibody targeting MB. Also known as Myoglobin (Muscle Cell Marker). Reactive in Human, Mouse, Rat, Canine. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Myoglobin (MB), Monoclonal Antibody
Full Product Name
Myoglobin (Muscle Cell Marker)
Product Gene Name
Product Synonym Names
MB; Myoglobin; PVALB
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
MB/2105
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Canine
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Myoglobin is a cytosolic oxygen-binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. Myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. In combination with other striated muscle markers such as vimentin and myogenin, myoglobin is helpful in the identification of rhabdomyosarcoma and tumors with skeletal muscle differentiation. Reportedly, myoglobin is expressed on epithelial cancer cells due to changed metabolic and environmental conditions.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
17kDa
3D Structure
ModBase 3D Structure for P02144
Immunogen
Recombinant human full-length Myoglobin (MB) protein.
Positive Control
SK-BR3 or T47D Cells. Skeletal or Cardiac Muscle
Research Areas
Cardiovascular, Hypoxia
Applications Tested/Suitable for anti-MB antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-MB antibody
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-MB antibody

Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Myoglobin Mouse Monoclonal Antibody (MB/2105).
Mouse Anti-MB Monoclonal Antibody – Cat# MBS4380618

Immunohistochemistry (IHC) of anti-MB antibody

Formalin-fixed, paraffin-embedded Mouse Heart stained with Myoglobin Mouse Monoclonal Antibody (MB/2105).
Mouse Anti-MB Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380618

Testing Data of anti-MB antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Myoglobin Mouse Monoclonal Antibody (MB/2105). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-MB Monoclonal Antibody, Testing Data – Cat# MBS4380618

Product References and Citations for anti-MB antibody

Qiu, Y., et al. 1998. Identification of myoglobin in human smooth muscle. J. Biol. Chem. 273: 23426-23432.

Other Notes

Small volumes of anti-MB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myoglobin
NCBI Official Synonym Full Names
myoglobin
NCBI Official Symbol
MB
NCBI Official Synonym Symbols
PVALB; myoglobgin
UniProt Protein Name
Myoglobin
Protein Family
UniProt Gene Name
MB
NCBI Protein Information
myoglobin

NCBI Summary for MB

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for MB

Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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