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S100A4 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380683

MBS4380683 is an antibody targeting S100A4. Also known as S100A4 (Marker of Tumor Metastasis). Reactive in Human, Mouse. Others not known.. Host: Rabbit. Recombinant Rabbit Monoclonal Antibody More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
S100A4, Monoclonal Antibody
Full Product Name
S100A4 (Marker of Tumor Metastasis)
Product Gene Name
Product Synonym Names
S100A4; S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein
Clonality
Monoclonal
Isotype
IgG / Kappa
Clone Number
S100A4/2750R
Host
Rabbit
Species Reactivity
Human, Mouse. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
10-12kDa
3D Structure
ModBase 3D Structure for P26447
Immunogen
Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary)
Positive Control
HeLa, A549 or A375 Cells. Placenta
Cellular Localization
Cytoplasm, Nucleus, Secreted
Applications Tested/Suitable for anti-S100A4 antibody
Flow Cytometry (Flow), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-S100A4 antibody
Flow: 1-2ug/million cells
IF: 1-3ug/ml
IHC: 1-2ug/ml; 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
WB: 2-4ug/ml

Immunofluorescence (IF) of anti-S100A4 antibody

Immunofluorescence staining of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.
Rabbit Anti-S100A4 Monoclonal Antibody – Cat# MBS4380683

Flow Cytometry (Flow) of anti-S100A4 antibody

Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).
Rabbit Anti-S100A4 Monoclonal Antibody, Flow Cytometry (Flow) – Cat# MBS4380683

SDS-PAGE of anti-S100A4 antibody

SDS-PAGE Analysis Purified S100A4Recombinant Rabbit Monoclonal Antibody (S100A4/2750R). Confirmation of Purity and Integrity of Antibody.
Rabbit Anti-S100A4 Monoclonal Antibody, SDS-PAGE – Cat# MBS4380683

Western Blot (WB) of anti-S100A4 antibody

Western Blot Analysis of Human Heart, Human Lung, Human Spleen, Human Ovary, A549, and HeLa lysates using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R)
Rabbit Anti-S100A4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380683

Western Blot (WB) of anti-S100A4 antibody

Western Blot Analysis of Heart, Lung and Spleen tissue lysates of different species using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).
Rabbit Anti-S100A4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380683

Immunohistochemistry (IHC) of anti-S100A4 antibody

Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).
Rabbit Anti-S100A4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380683

Product References and Citations for anti-S100A4 antibody

Ismail NI, Kaur G, Hashim H, Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int. 2008 Sep 5; 8:12.

Other Notes

Small volumes of anti-S100A4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for S100A4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein S100-A4
NCBI Official Synonym Full Names
S100 calcium binding protein A4
NCBI Official Symbol
S100A4
NCBI Official Synonym Symbols
42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
UniProt Protein Name
Protein S100-A4
UniProt Synonym Protein Names
Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
Protein Family
UniProt Gene Name
S100A4
UniProt Synonym Gene Names
CAPL; MTS1
NCBI Protein Information
protein S100-A4

NCBI Summary for S100A4

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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