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Spermidine or Spermine N1-Acetyltransferase 1 (SAT1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380686

MBS4380686 is an antibody targeting SAT1. Also known as Spermidine or Spermine N1-Acetyltransferase 1. Reactive in Human. Others not tested.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Spermidine or Spermine N1-Acetyltransferase 1 (SAT1), Monoclonal Antibody
Full Product Name
Spermidine or Spermine N1-Acetyltransferase 1
Product Gene Name
Product Synonym Names
DC21, Diamine acetyltransferase 1; Diamine N acetyltransferase 1; EC 2.3.1.57; KDSD; KSDX; Polyamine N acetyltransferase 1; Putrescine acetyltransferase; SAT1; spermidine/spermine N1 acetyltransferase alpha; SSAT; SSAT1
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CPTC-SAT1-3
Host
Mouse
Species Reactivity
Human. Others not tested.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
20kDa
3D Structure
ModBase 3D Structure for P21673
Immunogen
Recombinant human full-length SAT1 protein
Positive Control
Human liver tissue lysate; human tonsil or kidney.
Cellular Localization
Cytoplasm.
Chromosome Location
Xp22.11
Applications Tested/Suitable for anti-SAT1 antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-SAT1 antibody
Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Testing Data of anti-SAT1 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Spermidine Monoclonal Antibody (CPTC-SAT1-3).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-SAT1 Monoclonal Antibody – Cat# MBS4380686

Product References and Citations for anti-SAT1 antibody

Characterization of human spermidine/spermine N1-acetyltransferase purified from cultured melanoma cells. Libby, P.R., Ganis, B., Bergeron, R.J., Porter, C.W. Arch. Biochem. Biophys. (1991)

Other Notes

Small volumes of anti-SAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
diamine acetyltransferase 1
NCBI Official Synonym Full Names
spermidine/spermine N1-acetyltransferase 1
NCBI Official Symbol
SAT1
NCBI Official Synonym Symbols
SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
UniProt Protein Name
Diamine acetyltransferase 1
UniProt Synonym Protein Names
Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; Spermidine/spermine N(1)-acetyltransferase 1; SSAT; SSAT-1
UniProt Gene Name
SAT1
UniProt Synonym Gene Names
SAT; SSAT; SSAT-1
NCBI Protein Information
diamine acetyltransferase 1

NCBI Summary for SAT1

The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.[provided by RefSeq, Sep 2009]
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UniProt Comments for SAT1

Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N1-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N1- and N8-acetylspermidine), spermine, N1-acetylspermidine and N8-acetylspermidine.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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