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SOX9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380693

MBS4380693 is an antibody targeting SOX9. Also known as SOX9/SRY-box 9. Reactive in Human. Others not tested.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SOX9/SRY-box 9, Monoclonal Antibody
Full Product Name
SOX9/SRY-box 9
Product Gene Name
Product Synonym Names
Campomelic Dysplasia Autosomal Sex Reversal (CMD1); SRA1; SRXX2; SRY (sex determining region Y) box 9; SRY related HMG box gene 9; Transcription factor SOX9
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SOX9/2398
Host
Mouse
Species Reactivity
Human. Others not tested.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
65kDa
3D Structure
ModBase 3D Structure for P48436
Immunogen
Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary)
Positive Control
HepG2 Cells. Pancreas
Cellular Localization
Nuclear
Chromosome Location
17q24.3
Applications Tested/Suitable for anti-SOX9 antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-SOX9 antibody
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-SOX9 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with SOX9 Mouse Monoclonal Antibody (SOX9/2398).
Mouse Anti-SOX9 Monoclonal Antibody – Cat# MBS4380693

SDS-Page of anti-SOX9 antibody

SDS-PAGE Analysis Purified SOX9 Mouse Monoclonal Antibody (SOX9/2398). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-SOX9 Monoclonal Antibody, SDS-Page – Cat# MBS4380693

Testing Data of anti-SOX9 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX9 Mouse Monoclonal Antibody (SOX9/2398). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-SOX9 Monoclonal Antibody, Testing Data – Cat# MBS4380693

Product References and Citations for anti-SOX9 antibody

Zhu, H., et al. 2013. Upregulation of SOX9 in osteosarcoma and its association with tumor progression and patients prognosis. Diagn.Pathol. 8:183.

Other Notes

Small volumes of anti-SOX9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor SOX-9
NCBI Official Synonym Full Names
SRY-box 9
NCBI Official Symbol
SOX9
NCBI Official Synonym Symbols
CMD1; SRA1; CMPD1; SRXX2; SRXY10
UniProt Protein Name
Transcription factor SOX-9
Protein Family
UniProt Gene Name
SOX9
NCBI Protein Information
transcription factor SOX-9

NCBI Summary for SOX9

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]
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UniProt Comments for SOX9

Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development (PubMed:24038782). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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